UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot

文献类型： 外文期刊

第一作者： Wu, Xinxin

作者： Wu, Xinxin;Ni, Xiaopeng;Zhou, Yong;Gao, Zhihong;Wu, Xinxin;Gong, Qinghua

作者机构：

关键词： anthocyanins content;enzyme activity;Japanese apricot;RNA-seq;transcriptome analysis;UFGT;variegation;nonsynonymous mutation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Japanese apricot (Prunus mume Sieb. et Zucc.) is an important ornamental plant in China. One of the traits of petals color variegation is attractive, but its formation mechanism is unclear. In our study, RNA-seq technology was employed to characterize the transcriptome response to the mutation of "Fuban Tiaozhi" associated with petals variegation in Japanese apricot. As a result, 4,579,040 (white-flowered, WF) and 7,269,883 (red-flowered, RF) reads were mapped to P. persica genes, while 5,006,676 (WF) and 7,907,436 (RF) were mapped to P. persica genomes. There were 960 differentially expressed genes (DEGs) identified. Gene ontology analysis showed that these genes involved in 37 functional groups including 19 biological processes, 10 cellular components and eight molecular functions. Pathway enrichment annotation demonstrated that highly ranked genes were associated with flavonoid biosynthesis, anthocyanin biosynthesis, anthocyanins transports, plant hormone signal transduction, and transcriptional factors. The expression patterns part of them were validated by qRT-PCR. We found that UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene showed differential expression pattern. The UFGT enzyme activities in RF had a significantly higher than that of WF and lower in the initial stage and increased when the red appeared in the petals, which is identical to the accumulation of anthocyanins. And we also validated the SNPs, leading to the nonsynonymous mutations, in the UFGT by Sanger sequencing which may affect the enzyme activity. In summary, our results providemolecular candidates for better understanding themechanisms of the variegation in Japanese Apricot.

分类号：

  • 相关文献

[1]Physiological Investigation and Transcriptome Analysis of Polyethylene Glycol (PEG)-Induced Dehydration Stress in Cassava. Fu, Lili,Ding, Zehong,Han, Bingying,Hu, Wei,Li, Yajun,Zhang, Jiaming. 2016

[2]Mining for Candidate Genes in an Introgression Line by Using RNA Sequencing: The Anthocyanin Overaccumulation Phenotype in Brassica. Xie, Lulu,Li, Fei,Zhang, Shifan,Zhang, Hui,Qian, Wei,Li, Peirong,Zhang, Shujiang,Sun, Rifei. 2016

[3]Pistil abortion in Japanese apricot (Prunus mume Sieb. et Zucc.): isolation and functional analysis of PmCCoAOMT gene. Sun, Hailong,Shi, Ting,Song, Juan,Xu, Yanshuai,Gao, Zhihong,Song, Xinxin,Ni, Zhaojun,Cai, Binhua,Sun, Hailong.

[4]Comparative proteomic analysis of floral color variegation in peach. Zhou, Yong,Wu, Xinxin,Zhang, Zhen,Gao, Zhihong,Zhou, Yong.

[5]Comparative transcriptome analysis of cotton fiber development of Upland cotton (Gossypium hirsutum) and Chromosome Segment Substitution Lines from G. hirsutum x G. barbadense. Peng-tao Li,Mi Wang,Guo, Xiao-ping,Shi, Yu-zhen,Yuan, You-lu,Quan-wei Lu,Qun Ge,Md. Harun or Rashid,Ai-ying Liu,Ju-wu Gong,Hai-hong Shang,Wan-kui Gong,Jun-wen Li,Wei-wu Song,Li-xue Guo,Wei Su,Shao-qi Li,Xiao-ping Guo,Yu-zhen Shi,You-lu Yuan. 2017

[6]Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Quan Sun,Huaizhong Jiang,Xiaoyan Zhu,Weina Wang,Xiaohong He,Yuzhen Shi,Youlu Yuan,Xiongming Du,Yingfan Cai. 2013

[7]Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus. Ni, Zixin,Yang, Fan,Cao, Weijun,Zhang, Xiangle,Jin, Ye,Mao, Ruoqing,Du, Xiaoli,Li, Weiwei,Guo, Jianhong,Liu, Xiangtao,Zhu, Zixiang,Zheng, Haixue,Ni, Zixin. 2016

[8]Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Setting in Triploid Loquat (Eriobotrya japonica). Jiang, Shuang,Luo, Jun,Xu, Fanjie,Zhang, Xueying,Jiang, Shuang,Luo, Jun,Xu, Fanjie,Zhang, Xueying. 2016

[9]Genome-wide transcriptome analysis of Chinese pollination-constant nonastringent persimmon fruit treated with ethanol. Luo, Chun,Zhang, Qinglin,Luo, Zhengrong,Luo, Chun. 2014

[10]Transcriptome analysis of genes involved in anthocyanins biosynthesis and transport in berries of black and white spine grapes (Vitis davidii). Sun, Lei,Fan, Xiucai,Zhang, Ying,Jiang, Jianfu,Sun, Haisheng,Liu, Chonghuai. 2016

[11]The Rosa chinensis cv. Viridiflora Phyllody Phenotype Is Associated with Misexpression of Flower Organ Identity Genes. Yan, Huijun,Zhang, Hao,Wang, Qigang,Jian, Hongying,Qiu, Xianqin,Wang, Jihua,Tang, Kaixue,Baudino, Sylvie,Just, Jeremy,Raymond, Olivier,Bendahmane, Mohammed,Gu, Lianfeng. 2016

[12]Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight. Wang, Xu-Ming,Zhou, Jie,Yang, Yong,Yu, Fei-Bo,Chen, Juan,Yu, Chu-Lang,Wang, Fang,Cheng, Ye,Yan, Cheng-Qi,Chen, Jian-Ping,Yu, Fei-Bo,Chen, Juan. 2013

[13]Molecular identification of differential expression genes associated with sex pheromone biosynthesis in Spodoptera exigua. Zhang, Ya-Nan,Li, Jin-Bu,Zhu, Xiu-Yun,Zhang, Long-Wa,Chen, Da-Song,Sun, Liang,Li, Zhao-Qun,Ye, Zhan-Feng,Zheng, Mei-Yan.

[14]Transcriptomic Analysis for Different Sex Types of Ricinus communis L. during Development from Apical Buds to Inflorescences by Digital Gene Expression Profiling. Tan, Meilian,Xue, Jianfeng,Wang, Lei,Fu, Chunling,Yan, Xingchu,Huang, Jiaxiang. 2016

[15]Transcriptome analysis of pale-green leaf rice reveals photosynthetic regulatory pathways. Zhao, Xia,Feng, Baohua,Chen, Tingting,Zhang, Caixia,Tao, Longxing,Fu, Guanfu,Zhao, Xia. 2017

[16]Candidate chemosensory genes identified in Colaphellus bowringi by antennal transcriptome analysis. Li, Xiao-Ming,Zhu, Xiu-Yun,Wang, Zhi-Qiang,Wang, Yi,Chen, Geng,Deng, Dao-Gui,Zhang, Ya-Nan,He, Peng,Sun, Liang. 2015

[17]Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea). Xiao, Zhiliang,Hu, Yang,Zhang, Xiaoli,Xue, Yuqian,Fang, Zhiyuan,Yang, Limei,Zhang, Yangyong,Liu, Yumei,Li, Zhansheng,Liu, Xing,Liu, Zezhou,Lv, Honghao,Zhuang, Mu. 2017

[18]Comparative transcriptome profiling of genes and pathways related to resistance against powdery mildew in two contrasting melon genotypes. Zhu, Qianglong,Gao, Peng,Wan, Yan,Cui, Haonan,Fan, Chao,Liu, Shi,Luan, Feishi,Zhu, Qianglong,Gao, Peng,Wan, Yan,Cui, Haonan,Liu, Shi,Luan, Feishi,Fan, Chao. 2018

[19]Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages. Hao, Xinyuan,Yang, Yajun,Yue, Chuan,Wang, Lu,Wang, Xinchao,Hao, Xinyuan,Yang, Yajun,Yue, Chuan,Wang, Lu,Wang, Xinchao,Horvath, David P.. 2017

[20]Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.). Chen, Na,Chi, Xiaoyuan,Zhang, Zhimeng,Pan, Lijuan,Chen, Mingna,Wang, Tong,Wang, Mian,Yang, Zhen,Yu, Shanlin,Su, Maowen,Chi, Xiaoyuan.

作者其他论文 更多>>

微信小程序