Catecholamine-Stimulated Growth of Aeromonas hydrophila Requires the TonB2 Energy Transduction System but Is Independent of the Amonabactin Siderophore

文献类型: 外文期刊

第一作者: Dong, Yuhao

作者: Dong, Yuhao;Liu, Jin;Pang, Maoda;Du, Hechao;Wang, Nannan;Awan, Furqan;Lu, Chengping;Liu, Yongjie;Pang, Maoda

作者机构:

关键词: Aeromonas hydrophila;stress;catecholamine;TonB2 energy transduction system;amonabactin

期刊名称:FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY ( 影响因子:5.293; 五年影响因子:5.882 )

ISSN: 2235-2988

年卷期: 2016 年 6 卷

页码:

收录情况: SCI

摘要: The growth-stimulating effects of catecholamine stress hormones have been demonstrated in many pathogens. However, catecholamine-induced growth and its underlying mechanisms remain poorly understood in Aeromonas hydrophila . The present study sought to demonstrate that norepinephrine (NE), epinephrine (Epi), dopamine (Dopa), and L-dopa stimulate the growth of A. hydrophila in iron-restricted media containing serum. NE exhibited the strongest growth stimulation, which could be blocked by adrenergic antagonists. Furthermore, it was demonstrated that NE could sequester iron from transferrin, thereby providing a more accessible iron source for utilization by A. hydrophila . The deletion of the amoA gene associated with amonabactin synthesis revealed that the amonabactin siderophore is not required for NE-stimulated growth. However, the deletion of the TonB2 energy transduction system resulted in the loss of growth promotion by NE, indicating that a specific TonB-dependent outer membrane receptor might be involved in the transport of iron from transferrin. Collectively, our data show that catecholamine sensing promotes the growth of A. hydrophila in a manner that is dependent on the TonB2 energy transduction system

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