Soybean C2H2-Type Zinc Finger Protein GmZFP3 with Conserved QALGGH Motif Negatively Regulates Drought Responses in Transgenic Arabidopsis

文献类型: 外文期刊

第一作者: Zhang, Dayong

作者: Zhang, Dayong;Xu, Zhaolong;Wei, Peipei;Xu, Ling;Wan, Qun;Huang, Yihong;He, Xiaolan;Shao, Hongbo;Ma, Hongxiang;Tong, Jinfeng;Yang, Jiayin;Shao, Hongbo

作者机构:

关键词: Soybean;GmZFP3;transgenic Arabidopsis;drought response;negatively

期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.753; 五年影响因子:6.612 )

ISSN: 1664-462X

年卷期: 2016 年 7 卷

页码:

收录情况: SCI

摘要: Plant response to environmental stresses is regulated by a complicated network of regulatory and functional genes. In this study, we isolated the putative stress-associated gene GmZFP3 (a C2H2-type Zinc finger protein gene) based on the previous finding that it was one of two genes located in the QTL region between the Satt590 and Satt567 markers related to soybean tolerance to drought. Temporal and spatial expression analysis using quantitative real-time PCR indicated that GmZFP3 was primarily expressed in roots, stems and leaf organs and was expressed at low levels in flowers and soybean pods. Moreover, GmZFP3 expression increased in response to polyethylene glycol (PEG) and Abscisic acid (ABA) treatments. In addition, subcellular localization analysis indicated that GmZFP3 was ubiquitously distributed in plant cells. Transgenic experiments indicated that GmZFP3 played a negative role in plant tolerance to drought. Analysis of ABA-related marker gene expression in Arabidopsis suggested that GmZFP3 might be involved in the ABA-dependent pathway during the drought stress response. Taken together, these results suggest that soybean GmZFP3 negatively regulates the drought response.

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