An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea
文献类型： 外文期刊
第一作者： Ma, Duanzheng
作者： Ma, Duanzheng;Wang, He Xiang;Sekete, Malota;Ma, Duanzheng;Wang, He Xiang;Sekete, Malota;Wang, Bo;Gong, Zhiyuan;Ng, Tzi Bun
作者机构：
关键词： Purification; Mushroom; Lectin; Amanita; Agglutination
期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）
ISSN： 1359-5113
年卷期： 2014 年 49 卷 3 期
页码：
收录情况： SCI
摘要： A 37.4 kDa acid tolerant lectin was isolated and purified from dried fruiting bodies of Amanita hemibapha var. ochracea designated as AHL. The lectin was not adsorbed on DEAE-cellulose, but rather adsorbed on S-Sepharose and subjected to gel filtration by fast protein liquid chromatography on Superdex 75. The purified lectin was immune from inhibition activities of metal ions. More over, AHL exhibited high agglutination activity on rabbit erythrocytes with accelerating Hg2+ ions concentration. Partial peptide sequence analysis (VSNNLLTGPKVVR) of this lectin showed relative similarity to phosphoenolpyruvate carboxykinase [ATP]-like protein as predicted from Fragaria vesca subsp. Vesca. Interestingly, AHL displayed a strong affinity toward alpha-Lactose, making our study the first report associating Amanita species' lectin specificity for alpha-Lactose to the best of our knowledge. (C) 2014 Elsevier Ltd. All rights reserved.
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