Assessment of pulsed electric fields induced cellular damage in Saccharomyces cerevisiae: Change in performance of mitochondria and cellular enzymes
文献类型： 外文期刊
第一作者： Zhao, Wei
作者： Zhao, Wei;Yang, Ruijin;Tanga, Yali;Yang, Ruijin;Gu, Yan-jie;Tanga, Yali;Li, Chunyang
作者机构：
关键词： Pulsed electric fields (PEP);Non-thermal processing;Sublethal injury;Cellular enzymes
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）
ISSN： 0023-6438
年卷期： 2014 年 58 卷 1 期
页码：
收录情况： SCI
摘要： Pulsed electric fields (PEF) induced cellular damage in Saccharomyces cerevisiae, mainly focusing on metabolic properties including performance of mitochondria and cellular enzymes, was evaluated in this study. The proportion of sublethally injured S. cerevisiae cells was around 90% after PEF treatment at 20 kV/cm for 100-500 mu s. Sublethal injure S. cerevisiae correlated with damage to mitochondria and membranes caused by PEF, which was also confirmed by the glucose-energized repair process. An increase in plasma membrane Ca2+-ATPase, decrease of cytoplasmic esterase activity and changes of ten cellular enzymes of S. cerevisiae were observed after PEF treatment, suggesting the sublethal injury of S. cerevisiae is correlated with the inactivation or activation of cellular enzymes and aggregation of proteins under PEF treatment. (C) 2014 Elsevier Ltd. All rights reserved.
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