Characterization of target mRNAs for Prunus persica microRNAs using an integrated strategy of PLM-RACE, PPM-RACE and qRT-PCR

文献类型： 外文期刊

第一作者： Zhang, Yanping

作者： Zhang, Yanping;Han, Jian;Pervaiz, Tariq;Fang, Jinggui;Yu, Mingliang;Ma, Ruijuan

作者机构：

关键词： Ppe-miRNA;Target mRNA;PPM-RACE;RLM-RACE;Cleavage;qRT-PCR

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2014 年 170 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) in higher plants control their target mRNAs by either mRNA cleavage or translation repression at transcriptional or posttranscriptional levels. To verify more predicted target genes of Prunus persica microRNAs (Ppe-miRNAs) and elucidate the modes by which these Ppe-miRNAs work on their target genes, nine potential target genes (ppa005013m, ppa006611m, ppa007056m, ppa007653m, ppa001386m, ppa001781m, ppa003783m, ppa003465m, ppa004699m) for seven Ppe-miRNAs (PpemiR156a, Ppe-miR164a, Ppe-miR165a, Ppe-miR171a, Ppe-miR172a, Ppe-miR393a, Ppe-miR394a) were experimentally verified by a new strategy integrating modified 5' RLM-RACE (RNA ligase-mediated 5' rapid amplification of cDNA ends), 3' PPM-RACE (Poly(A) polymerase-mediated 3' rapid amplification of cDNA ends) and qRT-PCRs of cleavage products. The results show that these Ppe-miRNAs negatively regulate the expression of their target mRNAs by guiding cleavage of corresponding target mRNA with the cleavage sites mostly being at the tenth nucleotide of 5' end binding sites of Ppe-miRNAs. The expression patterns of Ppe-miRNAs, their target mRNAs and the cleavage fragments of target mRNAs were also analyzed in eight different peach tissues. There was an inverse relationship between expression of Ppe-miRNAs and their target mRNAs in eight tissues, and expression of both cleaved targets and Ppe-miRNAs verify the cleavage mode of Ppe-miRNAs on their target genes. Our results confirm the importance of Ppe-miRNAs in peach growth and development, and further increase understanding on the important regulatory roles of Ppe-miRNAs in peach, this suggest more study on Ppe-miRNAs and targets can enrich the knowledge of miRNA's functions in peach. (C) 2014 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[2]Characterization of target mRNAs for grapevine microRNAs with an integrated strategy of modified RLM-RACE, newly developed PPM-RACE and qPCRs. Wang, Chen,Han, Jian,Korir, Nicholas Kibet,Leng, Xiangpeng,Fang, Jinggui,Wang, Xicheng,Liu, Hong,Li, Xiaoying.

[3]Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. Wang, Sanhong,Yao, Quanhong,Tao, Jianmin,Qiao, Yushan,Zhang, Zhen.

[4]THERMOLYSIN DAMAGES ANIMAL LIFE THROUGH DEGRADATION OF PLASMA PROTEINS ENHANCED BY RAPID CLEAVAGE OF SERPINS AND ACTIVATION OF PROTEASES. Kong, Lulu,Liu, Chaoliang,Kong, Lulu,Kong, Lulu,Lu, Anrui,Guan, Jingmin,Yang, Bing,Ling, Erjun,Li, Muwang,Hillyer, Julian F.,Hillyer, Julian F.,Ramarao, Nalini,Soderhall, Kenneth.

[5]GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L). Fengli Zhao,JianhuiMa,Libei Li,Shuli Fan,Yaning Guo,Meizhen Song,Hengling Wei,Chaoyou Pang,Shuxun Yu. 2016

[6]SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water. Guan, Changfei,Chen, Li,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Liu, Jihong,Luo, Zhengrong,Du, Xiaoyun. 2015

[7]A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine in Catharanthus roseus. Gao, Fangyuan,Ren, Juansheng,Lu, Xianjun,Ren, Guangjun,Wang, Rui. 2017

[8]Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa). Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Ahmadi, Noorollah,Holloway, Patricia. 2014

[9]Molecular Cloning and Induced Expression of Six Small Heat Shock Proteins Mediating Cold-Hardiness in Harmonia axyridis (Coleoptera: Coccinellidae). Wang, Hui-Juan,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Cai-Di,Wang, Shi-Gui,Tang, Bin,Wang, Bing,Wang, Su,Meng, Zhao-Jun. 2017

[10]Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus. Zhang Bo,Song Wenping,Zheng Debin,Ma Chao,Xiao Guangxia,Wang Xianli. 2015

[11]Transcript Profiling of Hevea brasiliensis during Latex Flow. Tian, Wei-Min. 2017

[12]Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments. Niu, Xiaoping,Qi, Jianmin,Zhang, Gaoyang,Xu, Jiantang,Tao, Aifen,Fang, Pingping,Zhang, Gaoyang,Su, Jianguang. 2015

[13]Cloning and expression analysis of two different LhcSR genes involved in stress adaptation in an Antarctic microalga, Chlamydomonas sp ICE-L. Mou, Shanli,Liang, Qiang,Miao, Jinlai,Zheng, Zhou,Zhang, Xiaowen,Ye, Naihao,Xu, Dong,Dong, Meitao,Liang, Chengwei. 2012

[14]Molecular cloning and characterization of High Mobility Group box (HMGB) gene from Beauveria bassiana- infected silkworm, Bombyx mori. Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.,Chengxiang, H.,Han, W.,Dingding, L.,Ruilin, L.,Xijie, G.. 2017

[15]Selection of Suitable Reference Genes for Quantitative Real-time PCR in Sapium sebiferum. Chen, Xue,Mao, Yingji,Huang, Shengwei,Ni, Jun,Lu, Weili,Hou, Jinyan,Wang, Muting,Zhao, Weiwei,Li, Minghao,Wu, Lifang,Chen, Xue,Mao, Yingji,Huang, Shengwei,Ni, Jun,Lu, Weili,Hou, Jinyan,Wang, Muting,Zhao, Weiwei,Li, Minghao,Wu, Lifang,Chen, Xue,Mao, Yingji,Lu, Weili,Wang, Muting,Lu, Weili,Wang, Muting,Wang, Qiaojian. 2017

[16]Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR. Chi, Xiaoyuan,Yang, Qingli,Pan, Lijuan,Chen, Na,Chen, Mingna,Yang, Zhen,Wang, Tong,He, Yanan,Yu, Shanlin,Hu, Ruibo,Zhang, Xiaowen. 2012

[17]Expression Analysis of Anthocyanin Biosynthetic Genes in Different Colored Sweet Cherries (Prunus avium L.) During Fruit Development. Liu, Yun,Shen, Xinjie,Zhao, Kai,Guo, Xinwei,Li, Tianhong,Ben, Yue,Zhang, Xiaoming. 2013

[18]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

[19]Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis. Sun, M.,Jiang, K.,Zhang, F.,Zhang, D.,Shen, A.,Jiang, M.,Shen, X.,Ma, L.,Sun, M.,Zhang, D.. 2012

[20]Pathogenicity and tissue distribution of grass carp reovirus after intraperitoneal administration. Liang, Hong-Ru,Li, Yong-Gang,Zeng, Wei-Wei,Wang, Ying-Ying,Wang, Qing,Wu, Shu-Qin,Li, Yong-Gang. 2014

作者其他论文 更多>>

微信小程序