Effects of pulsed electric fields on cytomembrane lipids and intracellular nucleic acids of Saccharomyces cerevisiae

文献类型： 外文期刊

第一作者： Zhao, Wei

作者： Zhao, Wei;Yang, Ruijin;Gu, Yanjie;Zhao, Wei;Yang, Ruijin;Gu, Yanjie;Li, Chunyang

作者机构：

关键词： Pulsed electric fields (PEF);Sublethal injury;Cytomembrane;Nucleic acids

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2014 年 39 卷

页码：

收录情况： SCI

摘要： In this study, pulsed electric fields (PEF) treatments at 20 kV/cm for 0-500 mu s were applied to Saccharomyces cerevisiae to investigate the effects of PEF on the cytomembrane and intracellular nucleic acids. The change in composition of membrane lipids characterized by decrease in the ratio of contents of unsaturated fatty acids versus saturated fatty acids induced by PEF was observed, which was related with the increase in rigidness of cytomembrane (lower cytomembrane fluidity and higher cytomembrane viscosity) after PEE PEF treatment caused no significant change in DNA but disruption of RNA of S. cerevisiae, which was also confirmed by the increase of PEF resistance and no occurrence of injured microbial cells by supplementation of RNA stabilizer, magnesium. The results showed the damage of cytomembrane and RNA were associated with the sublethally injured S. cerevisiae, suggesting cytomembrane and RNA were primary targets for PEF induced microbial damage. (C) 2013 Elsevier Ltd. All rights reserved.

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