Detection of border disease virus (BDV) in goat herds suffering diarrhea in eastern China

文献类型: 外文期刊

第一作者: Li, Wenliang

作者: Li, Wenliang;Mao, Li;Zhao, Yongqian;He, Kongwang;Jiang, Jieyuan;Sun, Yinhua

作者机构:

关键词: Border disease virus;Goat;Diarrhea;Phylogenetic analysis;Eastern China

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN: 1743-422X

年卷期: 2013 年 10 卷

页码:

收录情况: SCI

摘要: Background: Border disease virus (BDV) is an important pathogen in sheep and goat production. Neither epidemiological investigation nor any reports of BDV infection was available in China. During Jan to Apr, 2012, several herd goats in Anhui and Jiangsu provinces in eastern China suffered unremitting diarrhea, with morbidity and mortality of about 28-37% and 10-15%, respectively. In the present study, sera and tissue samples from diseased goats of four farms were taken for BDV detection, isolation and identification. Results: Panpesti generic primers and border disease virus (BDV)-specific primers targeting the 5'-UTR region produced RT-PCR positive bands for sera (24/28) and tissue samples (7/30). Twenty positive sera and tissue samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells for virus isolation. Finally, three different strains of BDV, named AH12-01, AH12-02 and JS12/04, were successfully isolated as identified by RT-PCR using 5'-UTR and N-pro gene primers, sequencing and electron microscopy. Sequences of 5'-UTR and N-pro genes of them were used for phylogenetic analysis and comparison to other reference sequences available in GenBank. The results indicated AH12-01, AH12-02 and JS12/04 possess high relationship with the BDV 3 group viruses and differed with each other. Conclusion: This is the first detection of BDV from goats with diarrhea and confirmation of BDV infection in China.

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