Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase alpha 2 subunit

文献类型： 外文期刊

第一作者： Lin, Dingbo

作者： Lin, Dingbo;He, Hui;Willard, Lloyd;Ji, Hua;Ji, Hua;Willis, Jordan;Jiang, Yu;Wark, Logan;Han, Jing;Medeiros, Denis M.;Liu, Yongzhang;Lu, Bin;Liu, Yongzhang;Lu, Bin

作者机构：

关键词： AMP-activated protein kinase 2;Hepatic steatosis;Mitochondrial biogenesis;Mitophagy;Wolfberry

期刊名称：MOLECULAR NUTRITION & FOOD RESEARCH （ 影响因子：5.914； 五年影响因子：6.409 ）

ISSN： 1613-4125

年卷期： 2014 年 58 卷 5 期

页码：

收录情况： SCI

摘要： ScopeThe aim of this study is to investigate whether AMP-activated protein kinase 2 (AMPK2) is essential for wolfberry's protective effects on mitochondrial dysfunction and subsequent hepatic steatosis in mice. Methods and resultsSix-week-old male AMPK2 knockout mice and genetic background C57BL/6J (B6) mice were fed a control, high-fat diet (HD, 45% (kilocalorie) fat), and/or HD with 5% (kilocalarie) wolfberry diets for 18 wk. At termination, blood and liver tissues were sampled for analysis by ELISA, HPLC, microscopy, real-time PCR, and Western blot. HD lowered hepatic lutein and zeaxanthin contents, inhibited protein expression of ,-carotene 9,10-oxygenase 2 (BCO2) and heat shock protein 60 in mitochondria, increased reactive oxygen species level, and suppressed mitophagy and mitochondrial biogenesis as determined by accumulation of p62, inhibited phosphorylation of Unc-51-like kinase 1 on Ser555, and declined expression of peroxisome proliferator-activated receptor coactivator 1 , resulting in hepatic steatosis in B6 and knockout mice. Dietary wolfberry elevated the xanthophyll concentrations and enhanced expression of BCO2 and heat shock protein 60, attenuated mitochondrial oxidative stress, activated AMPK2, potentiated mitophagy and mitochondrial biogenesis, and enhanced lipid oxidation and secretion in the liver of B6 mice. ConclusionDietary wolfberry selectively activated AMPK2, which resulted in enhanced mitochondrial biogenesis and potentiated mitophagy, leading to the prevention of hepatic steatosis in obese mice.

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