Fermentation properties of isomaltooligosaccharides are affected by human fecal enterotypes

文献类型： 外文期刊

第一作者： Wu, Qinqin

作者： Wu, Qinqin;Wu, Qinqin;Pi, Xiong'e;Liu, Wei;Chen, Huahai;Yin, Yeshi;Wang, Xin;Zhu, Liying;Yu, Hongwei D.;Yu, Hongwei D.

作者机构：

关键词： Human gut microbiota;IMO;Degree of polymerization;Enterotype;SCFAs

期刊名称：ANAEROBE （ 影响因子：3.331； 五年影响因子：3.539 ）

ISSN： 1075-9964

年卷期： 2017 年 48 卷

页码：

收录情况： SCI

摘要： Isomaltooligosaccharides (IMOs) are enzymatically synthesized oligosaccharides that have potential prebiotic effects. Five IMO substrates with 2-16 of polymerization (DP) were studied for their fermentation capacities using human microbiomes in an in vitro batch fermentation model. Eleven fecal slurries belonging to three enterotypes, including the Bacteroides-, Prevotella- and Mixed -type, exhibited different degradation rates for long chain IMOs (DP 7 to 16). In contrast, the degradation rates for short chain IMOs (DP 2 to 6) were not affected by enterotypes. Both 16S rRNA gene sequencing and quantitative PCR demonstrated that, after fermentation, the Bifidobacterium growth with IMOs was primarily detected in the Bacteroides- and Mixed -type (non-Prevotella-type), and to a lesser degree in the Prevotella-type. Interestingly, the Prevotella-type microbiome had higher levels of propionic acid and butyric acid production than non-Prevotella-type microbiome after IMOs fermentation. Moreover, principal coordinate analysis (PCoA) of both denaturing gradient gel electrophoresis (DGGE) profiling and 16S rRNA sequencing data demonstrated that the microbiome community compositions were separately clustered based on IMO chain length, suggesting significant impact of DP on the bacterial community structure. The current results clearly demonstrated that the IMO chain length could modulate the structure and composition of the human colonic microbiome. Different responses to short and long chain IMOs were observed from three human enterotypes, indicating that IMOs may be used as therapeutic substrates for directly altering human colonic bacteria. (C) 2017 Elsevier Ltd. All rights reserved.

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