Papaya CpEIN3a and CpNAC2 Co-operatively Regulate Carotenoid Biosynthesis-Related Genes CpPDS2/4, CpLCY-e and CpCHY-b During Fruit Ripening

文献类型： 外文期刊

第一作者： Fu, Chang-chun

作者： Fu, Chang-chun;Kuang, Jian-fei;Chen, Jian-ye;Lu, Wang-jin;Han, Yan-chao

作者机构：

关键词： Carotenoid biosynthesis-related genes;EIN3;NAC;Papaya fruit;Transcriptional regulation

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2017 年 58 卷 12 期

页码：

收录情况： SCI

摘要： Papaya is an important tropical fruit with a rich source of carotenoids. The ripening of papaya is a physiological and metabolic process with remarkable changes including accumulation of carotenoids, which depends primarily on the action of ethylene. Ethylene response is mediated by a transcriptional cascade involving the transcription factor families of EIN3/EILs and ERFs. Although ERF members have been reported to control carotenoid production in Arabidopsis and tomato, whether EIN3/EILs are also involved in carotenoid biosynthesis during fruit ripening remains unclear. In this work, two EIN3 genes from papaya fruit, namely CpEIN3a and CpEIN3b, were studied, of which CpEIN3a was increased during fruit ripening, concomitant with the increase of transcripts of carotenoid biosynthesis related genes including CpPDS2/4, CpZDS, CpLCY-e and CpCHY-b, and carotenoid content. Electrophoretic mobility shift assays (EMSAs) and transient expression analyses revealed that CpEIN3a was able to bind to the promoters of CpPDS4 and CpCHY-b, and promoted their transcription. Protein-protein interaction assays indicated that CpEIN3a physically interacted with another transcription factor CpNAC2, which acted as a transcriptional activator of CpPDS2/4, CpZDS, CpLCY-e and CpCHY-b by directly binding to their promoters. More importantly, the transcriptional activation abilities of CpPDS2/4, CpLCY-e and CpCHY-b were more pronounced following their interaction. Collectively, our findings suggest that CpEIN3a interacts with CpNAC2 and, individually or co-operatively, activates the transcription of a subset of carotenoid biosynthesis related genes, providing new insights into the regulatory networks of carotenoid biosynthesis during papaya fruit ripening.

分类号：

  • 相关文献

[1]Transcription Factor AtMYB44 Regulates Induced Expression of the ETHYLENE INSENSITIVE2 Gene in Arabidopsis Responding to a Harpin Protein. Liu, Ruoxue,Chen, Lei,Jia, Zhenhua,Lue, Beibei,Shi, Haojie,Dong, Hansong,Liu, Ruoxue,Shao, Weilan,Chen, Lei,Jia, Zhenhua.

[2]The abiotic stress-responsive NAC transcription factor SlNAC11 is involved in drought and salt response in tomato (Solanum lycopersicum L.). Lingling Wang,Chen, Guoping,Zongli Hu,Mingku Zhu,Zhiguo Zhu,Jingtao Hu,Ghulam Qanmber,Guoping Chen.

[3]Analyses of the NAC Transcription Factor Gene Family in Gossypium raimondii Ulbr.: Chromosomal Location, Structure, Phylogeny, and Expression Patterns. Haihong Shang ,Wei Li,Changsong Zou,Youlu Yuan. 2013

[4]A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis. Lu, Min,Ying, Sheng,Zhang, Deng-Feng,Shi, Yun-Su,Song, Yan-Chun,Wang, Tian-Yu,Li, Yu. 2012

[5]NAC transcription factors in plant multiple abiotic stress responses: progress and prospects. Shao, Hongbo,Shao, Hongbo,Shao, Hongbo,Wang, Hongyan,Tang, Xiaoli,Wang, Hongyan. 2015

[6]Wheat NAC transcription factor TaNAC29 is involved in response to salt stress. Xu, Zhongyang,Gongbuzhaxi,Wang, Changyou,Zhang, Hong,Ji, Wanquan,Xu, Zhongyang,Xue, Fei.

[7]Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening. Kuang, Jian-fei,Chen, Lei,Xie, Hui,Peng, Huan-huan,Xiao, Yun-yi,Li, Xue-ping,Chen, Wei-xin,Chen, Jian-ye,Lu, Wang-jin,He, Quan-guang. 2012

[8]Characterization and primary functional analysis of a bamboo NAC gene targeted by miR164b. Wang, Lili,Zhao, Hansheng,Chen, Dongliang,Li, Lichao,Sun, Huayu,Lou, Yongfeng,Gao, Zhimin,Wang, Lili,Chen, Dongliang.

[9]Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp chinensis). Wang, Aihua,Huang, Xingxue,Li, Xia,Zhou, Guolin,Hu, Jihong,Hu, Jihong,Yan, Zhixiang. 2016

[10]Analyses of the NAC Transcription Factor Gene Family in Gossypium raimondii Ulbr.: Chromosomal Location, Structure, Phylogeny, and Expression Patterns. Shang, Haihong,Li, Wei,Zou, Changsong,Yuan, Youlu. 2013

[11]Identification of 7 stress-related NAC transcription factor members in maize (Zea mays L.) and characterization of the expression pattern of these genes. Lu, Min,Sun, Qing-Peng,Pan, Jin-bao,Zhang, Deng-feng,Wang, Tian-Yu.

[12]Genome-wide characterization and expression profiling of the NAC genes under abiotic stresses in Cucumis sativus. Zhang, Xiao Meng,Yu, Hong Jun,Sun, Chao,Deng, Jie,Zhang, Xue,Liu, Peng,Li, Yun Yun,Li, Qiang,Jiang, Wei Jie,Sun, Chao,Jiang, Wei Jie.

[13]The miR-302/367 cluster: a comprehensive update on its evolution and functions. Gao, Zeqian,Zhu, Xueliang,Dou, Yongxi. 2015

[14]Expression of TERF1 in rice regulates expression of stress-responsive genes and enhances tolerance to drought and high-salinity. Gao, Shumei,Zhang, Haiwen,Tian, Yun,Li, Fang,Zhang, Zhijin,Chen, Xiaoliang,Huang, Rongfeng,Gao, Shumei,Zhang, Haiwen,Zhang, Zhijin,Huang, Rongfeng,Tian, Yun,Li, Fang,Lu, Xiangyang. 2008

[15]SUV39H1 mediated SIRT1 trans-repression contributes to cardiac ischemia-reperfusion injury. Yang, Guang,Zhang, Xinjian,Liang, Peng,Dai, Xin,Zeng, Sheng,Xu, Huihui,Fang, Mingming,Li, Yuehua,Xu, Dachun,Xu, Yong,Weng, Xinyu,Huan, Hailin,Xu, Dachun. 2017

[16]Transcriptional and post-transcriptional regulation of the jasmonate signalling pathway in response to abiotic and harvesting stress in Hevea brasiliensis. Pirrello, Julien,Leclercq, Julie,Dessailly, Florence,Rio, Maryannick,Piyatrakul, Piyanuch,Montoro, Pascal,Piyatrakul, Piyanuch,Kuswanhadi, Kuswanhadi,Tang, Chaorong. 2014

[17]Low-Temperature-Induced Expression of Rice Ureidoglycolate Amidohydrolase is Mediated by a C-Repeat/Dehydration-Responsive Element that Specifically Interacts with Rice C-Repeat-Binding Factor 3. Li, Juan,Qin, Rui-Ying,Li, Hao,Xu, Rong-Fang,Yang, Ya-Chun,Ni, Da-Hu,Ma, Hui,Li, Li,Wei, Peng-Cheng,Yang, Jian-Bo. 2015

[18]Quantitative measurement of pH influence on SalR regulated gene expression in Acinetobacter baylyi ADP1. Li, Chao,Zhou, Lin,Zhang, Yongjun,Song, Fuping,Zhang, Jie.

[19]Transcriptional regulation of the gene for prothoracicotropic hormone in the silkworm, Bombyx mori. Wei, Zhao-Jun,Yu, Miao,Hong, Gui-Yun,Jiang, Shao-Tong,Tang, Shun-Ming,Yi, Yong-Zhu.

[20]Similar regulation of two distinct UL24 promoters by regulatory proteins of equine herpesvirus type 1 (EHV-1). Ma, Yue,Liu, Diqiu,Wang, Xiaojun,Gao, Jun,Wang, Xiaojun.

作者其他论文 更多>>

微信小程序