The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum

文献类型： 外文期刊

第一作者： Zhang, Xiaoping

作者： Zhang, Xiaoping;Chen, Xiang;Yu, Menghao;Yin, Yanni;Ma, Zhonghua;Jiang, Jinhua

作者机构：

关键词： TBCA;Conidiation;Cold sensitivity;F. asiaticum;Hyphal growth;Fusarium head blight

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2015 年 15 卷

页码：

收录情况： SCI

摘要： Background: Tubulin cofactor A ( TBCA), one of the members of tubulin cofactors, is of great importance in microtubule functions through participating in the folding of alpha/beta-tubulin heterodimers in Saccharomyces cerevisiae. However, little is known about the roles of TBCA in filamentous fungi. Results: In this study, we characterized a TBCA orthologue FaTBCA in Fusarium asiaticum. The deletion of FaTBCA caused dramatically reduced mycelial growth and abnormal conidiation. The FaTBCA deletion mutant (Delta FaTBCA-3) showed increased sensitivity to low temperatures and even lost the ability of growth at 4 degrees C. Microscopic observation found that hyphae of Delta FaTBCA-3 exhibited blebbing phenotypes after shifting from 25 to 4 degrees C for 1-or 3-day incubation and approximately 72% enlarged nodes contained several nuclei after 3-day incubation at 4 degrees C. However, hyphae of the wild type incubated at 4 degrees C were phenotypically indistinguishable from those incubated at 25 degrees C. These results indicate that FaTBCA is involved in cell division under cold stress (4 degrees C) in F. asiaticum. Unexpectedly,Delta FaTBCA-3 did not exhibit increased sensitivity to the anti-microtubule drug carbendazim although quantitative real-time assays showed that the expression of Delta FaTBCA was up-regulated after treatment with carbendazim. In addition, pathogenicity assays showed that Delta FaTBCA-3 exhibited decreased virulence on wheat head and on non-host tomato. Conclusion: Taken together, results of this study indicate that FaTBCA plays crucial roles in vegetative growth, conidiation, temperature sensitivity and virulence in F. asiaticum.

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