Molecular analysis of barley yellow mosaic virus isolates from China

文献类型: 外文期刊

第一作者: Chen, J

作者: Chen, J;Shi, NN;Cheng, Y;Diao, A;Chen, JP;Wilson, TMA;Antoniw, JF;Adams, MJJ

作者机构:

关键词: barley yellow mosaic virus;nucleotide sequence;isolate differences;single-strand conformation polymorphism

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN: 0168-1702

年卷期: 1999 年 64 卷 1 期

页码:

收录情况: SCI

摘要: The complete sequences of both RNAs of an isolate of barley yellow mosaic virus from Yancheng, Jiangsu province, China, were determined. The sequences resembled those of an isolate from Japan (96.8% identical nucleotides for RNA1; 95.7% for RNA2) more closely than one from Germany (93.9 and 91.0%, respectively). The greatest differences between the Chinese and Japanese isolates were in the 5'-UTRs of RNAs 1 and 2 (88.9 and 91.6% identical nucleotides, respectively) and there were also some other regions of difference in P1 (RNA2) and P3, CI, NIa and the 5' end of the coat protein (CP) (RNA1). Molecular differences between isolates from ten sites widely distributed in Eastern China were studied by sequencing RNA regions coding for the CP (RNA1) and the N-terminus of the P2 protein (RNA2). The P2 fragment was more variable than the CP, and phylogenetic analysis of both regions showed that Asian and European isolates formed distinct clusters. Differences between isolates were also revealed by single-strand conformation polymorphism of reverse transcription-polymerase chain reaction products, spanning the full lengths of both RNA1 and RNA2. However, molecular variations between isolates could not be linked to earlier results showing differences in cultivar response. (C) 1999 Elsevier Science B.V. All rights reserved.

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