MOLECULAR CHARACTERIZATION OF UK ISOLATES OF BARLEY YELLOW MOSAIC BYMOVIRUS

文献类型： 外文期刊

第一作者： SHI, NN

作者： SHI, NN;ZHU, MY;CHEN, JP;STRATFORD, R;WILSON, TMA;ANTONIW, JF;FOULDS, IJ;MACFARLANE, SA;ADAMS, MJ

作者机构：

关键词： BARLEY YELLOW MOSAIC VIRUS;RESISTANCE-BREAKING STRAIN;ISOLATE DIFFERENCE

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 1995 年 38 卷 2-3 期

页码：

收录情况： SCI

摘要： Several isolates of barley yellow mosaic virus (BaYMV) from different sites in the UK, including some that were virulent on European resistant winter barley cultivars (resistance-breaking strain: BaYMV-2) and some that were not, were examined by RT-PCR, restriction mapping and sequencing of selected parts of the virus genome. Nucleotide and predicted amino acid sequences were determined for the 5'-terminal region, part of the NIa coding region and the coat protein coding region on RNA 1 and an area at the N-terminus of the 70-kDa protein coding region on RNA 2. The sequences differed from those previously reported for a BaYMV isolate from Japan and for two German isolates, one of which was of the BaYMV-2 strain. There were no strain-specific amino acid differences and the few, non-consecutive, nucleotide differences detected were probably not significant and were insufficient to develop a rapid diagnostic test to distinguish BaYMV-2 from other isolates. Restriction mapping of RNA 2 cDNA again showed no consistent strain-related differences. The differences previously reported between the two German isolates are probably not strain-related.

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[1]Molecular analysis of barley yellow mosaic virus isolates from China. Chen, J,Shi, NN,Cheng, Y,Diao, A,Chen, JP,Wilson, TMA,Antoniw, JF,Adams, MJJ. 1999

[2]SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF RT-PCR PRODUCTS OF UK ISOLATES OF BARLEY YELLOW MOSAIC VIRUS. Shi, NN,Chen, JP,Wilson, TMA,Macfarlane, SA,Antoniw, JF,Adams, MJ.

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