Toxicity and affecting factors of Bacillus thuringiensis var. israelensis on Chironomus kiiensis larvae

文献类型： 外文期刊

第一作者： Cao, Chuan-Wang

作者： Cao, Chuan-Wang;Sun, Li-Li;Wen, Rong-Rong;Li, Xiao-Peng;Wang, Zhi-Ying;Wu, Hong-Qu

作者机构：

关键词： bioassay

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2012 年 12 卷

页码：

收录情况： SCI

摘要： Bacillus thuringiensis var. israelensis (Bti) is a suitable agent for controlling Chironomus kiiensis, a major pest polluting water. In this study, laboratory bioassays were used to study toxicity and affecting factors of Bti on C. kiiensis larvae. Tests were conducted using three commercial Bti formulations (oil miscible suspension, 1,200 ITU/mL; wettable power, 1,200 ITU/mg; technical material, 5,000 ITU/mg) of Bti. The toxicity of Bti formulations to third and fourth instar C. kiiensis larvae was in decreasing order of technical material, oil miscible suspension, and wettable powder, based on the 12 and 24 hour LC50 values. Increasing larval densities (from 10 to 30 per bioassay cup) increased the LC50 values for fourth instar C. kiiensis larvae. The LC50 values for fourth instar larvae reared in sand substrate were higher than those from soil substrate, and autoclaved substrates significantly increased the LC50 values. The technical material of Bti at 12 and 24 hours responded similarly to changes in temperature between 30 degrees C and 15 degrees C, but the LC50 values at a range of tested temperatures showed distinct differences in time points.

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