Expression of CP:CBF3-35S:ICE1 enhances low temperature tolerance in transgenic Arabidopsis

文献类型： 外文期刊

第一作者： Li, Ruimei

作者： Li, Ruimei;Duan, Ruijun;Liu, Jiao;Fu, Shaoping;Guo, Jianchun;Li, Ruimei;Duan, Ruijun;Liu, Jiao;Fu, Shaoping;Guo, Jianchun;Xi, Dujuan;Ji, Yimeng;Hu, Xinwen;Guo, Jianchun

作者机构：

关键词： CBF3;ICE1;cold resistance

期刊名称：ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6

ISSN： 1022-6680

年卷期： 2013 年 726-731 卷

页码：

收录情况： SCI

摘要： We have constructed a vector pCAMBIA1300-CP:CBF3-35S:ICE1 and transformed into Arabidopsis. Results of PCR proved that the target genes had integrated into Arabidopsis genome. Transgenic Arabidopsis showed a bit slow growth, earlier flowering, but normal at other phenotype under 22 degrees C with 8 h daily lights. In vitro low temperature stress tests showed that the transgenic lines were survival while the wild type was nearly dead. The transgenic plants also showed an increased proline content, SOD and POD activities under low temperature stress. The phenotype and physical evidence indicated that expression of CP:CBF3-358:ICE1 under low temperature enhances the cold tolerance in transgenic plants.

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