Identification and expression analysis of WRKY transcription factor genes in response to fungal pathogen and hormone treatments in apple (Malus domestica)

文献类型： 外文期刊

第一作者： Lui, Shuai

作者： Lui, Shuai;Zhu, Longming;Sha, Renhe;Qu, Shenchun;Cai, Binhua;Wang, Sanhong;Luo, Changguo

作者机构：

关键词： Alternaria alternata apple pathotype;Apple;Bioinformatics analysis;Expression profile analysis;Hormone treatment;WRKY transcription factor

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

ISSN： 1226-9239

年卷期： 2017 年 60 卷 2 期

页码：

收录情况： SCI

摘要： The WRKY family, a large family of transcription factors (TFs) found in higher plants, plays central roles in many aspects of biological processes and adaption to environment. However, little information is available on this family in apple (Malus domestica). In the present study, a total of 119 candidate WRKY genes in apple genome were identified and classified into three main groups (Group I-III) based on the structure of the conserved domains. Each group or subgroup showed similar exon-intron structures and motif compositions. The evolution analysis showed that 44 MdWRKY genes clustered into 20 intensive regions (<100 kb) and 78 MdWRKY formed 85 pairs of collinear relationships, suggesting that both tandem and segmental duplications played an important role in the evolution and diversification of the WRKY gene family in apple. Furthermore, the expression of the MdWRKY genes in apple leaves in response to biotic stress (Alternaria alternate) and hormone treatments [salicylic acid (SA), methyl jasmonate (MeJA) and ethephon] was examined by using RNA-seq and qRT-PCR. The results showed that 63 MdWRKY genes had differential expression in their transcript abundance in response to Alternaria alternata apple pathotype infection. Moreover, most pathogen responsive MdWRKY genes were also changed significantly when apple leaves were treated by SA, MeJA or ethephon plant growth regulations, suggesting an interaction between SA, JA and ethylene (Eth) hormone signaling under biotic stress. This work may provide the basis for future studies of the genetic modification of WRKY genes for pathogen resistance in apple.

分类号：

  • 相关文献

[1]Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS-LRR Protein Class Gene in Apple (Golden Delicious). Ma, Chao,Lu, You,Bai, Songlin,Zhang, Wennan,Duan, Xuwei,Meng, Dong,Wang, Zhigang,Li, Tianzhong,Wang, Zhigang,Zhou, Zongshan,Wang, Aide. 2014

[2]Genome-wide identification and evolutionary and expression analyses of MYB-related genes in land plants. Du, Hai,Wang, Yong-Bin,Xie, Yi,Zhang, Shuang-Shuang,Huang, Yu-Bi,Du, Hai,Tang, Yi-Xiong,Liang, Zhe,Jiang, San-Jie.

[3]Production of peanut hybrid seeds in an intersectional cross through post-pollination treatment of flower bases with plant growth regulators. Wang, Chuan Tang,Yu, Hong Tao,Wang, Xiu Zhen,Tang, Yue Yi,Wu, Qi,Song, Guo Sheng,Yu, Shan Lin,Gao, Hua Yuan,Hu, Dong Qing,Chen, Jian Hong.

[4]Genome-wide analysis of the WRKY gene family in cotton. Dou, Lingling,Zhang, Xiaohong,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Yu, Shuxun.

[5]Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment. Mao, Yizhou,Jiang, Biao,Peng, Qingwu,Liu, Wenrui,Lin, Yue,Xie, Dasen,He, Xiaoming,Mao, Yizhou,Jiang, Biao,Peng, Qingwu,Liu, Wenrui,Lin, Yue,Xie, Dasen,He, Xiaoming,Mao, Yizhou,Li, Shaoshan. 2017

[6]Characterization of HbWRKY1, a WRKY transcription factor from Hevea brasiliensis that negatively regulates HbSRPP. Wang, Ying,Guo, Dong,Li, Hui-Liang,Peng, Shi-Qing,Wang, Ying,Peng, Shi-Qing. 2013

[7]Genome-Wide Identification and Expression Analysis of the WRKY Gene Family in Cassava. Wei, Yunxie,Shi, Haitao,Xia, Zhiqiang,Tie, Weiwei,Ding, Zehong,Yan, Yan,Wang, Wenquan,Hu, Wei,Li, Kaimian. 2016

[8]Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean. Wu, Jing,Wang, Lanfen,Wang, Shumin,Chen, Jibao. 2016

[9]Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis. He, Guan-Hua,Xu, Ji-Yuan,Liu, Jia-Ming,Li, Pan-Song,Chen, Ming,Ma, You-Zhi,Xu, Zhao-Shi,Wang, Yan-Xia. 2016

[10]Genome-wide identification and characterization of WRKY gene family in Hevea brasiliensis. Li, Hui-Liang,Guo, Dong,Yang, Zi-Ping,Tang, Xiao,Peng, Shi-Qing,Yang, Zi-Ping.

[11]Regulation of cotton (Gossypium hirsutum) drought responses by mitogen-activated protein (MAP) kinase cascade-mediated phosphorylation of GhWRKY59. Li, Fangjun,Li, Maoying,Duan, Liusheng,Li, Zhaohu,Li, Fangjun,Wang, Ping,Cox, Kevin L., Jr.,Shan, Libo,Li, Maoying,He, Ping,Li, Maoying,Wang, Ping,Dever, Jane K..

[12]Identification and expression profiles of the WRKY transcription factor family in Ricinus communis. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing,Zhang, Liang-Bo,Li, Chang-Zhu.

[13]GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L). Fengli Zhao,JianhuiMa,Libei Li,Shuli Fan,Yaning Guo,Meizhen Song,Hengling Wei,Chaoyou Pang,Shuxun Yu. 2016

[14]Genome-wide identification and analysis of FK506-binding protein family gene family in strawberry (Fragaria x ananassa). Leng, Xiangpeng,Liu, Dan,Sun, Xin,Li, Yu,Mu, Qian,Zhu, Xudong,Li, Pengyu,Fang, Jinggui,Zhao, Mizhen. 2014

[15]Bioinformatic prediction and analysis of glucolipid metabolic regulation by miR-34a in Megalobrama amblycephala. Miao, Ling-Hong,Pan, Wen-Jing,Ge, Xian-Ping,Miao, Ling-Hong,Lin, Yan,Ge, Xian-Ping,Liu, Bo,Ren, Ming-Chun,Zhou, Qun-Lan,Miao, Ling-Hong,Pan, Wen-Jing,Lin, Yan,Ge, Xian-Ping,Liu, Bo,Ren, Ming-Chun,Zhou, Qun-Lan.

[16]Molecular cloning and expression analysis of the STAT1 gene in the water buffalo (Bubalus bubalis). Deng, Tingxian,Pang, Chunying,Zhu, Peng,Yang, Bingzhuang,Liang, Xianwei,Deng, Tingxian,Pang, Chunying,Zhu, Peng,Yang, Bingzhuang,Liang, Xianwei,Liao, Biyun,Zhang, Ming.

[17]Toxoplasma gondii: Bioinformatics analysis, cloning and expression of a novel protein TgIMP1. Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhou, Huaiyu,Cong, Hua,Zhao, Qunli,Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhu, Xing-Quan,Zhu, Xing-Quan.

[18]Bioinformatic analysis of gene encoding odorant binding protein (OBP) 1, OBP2, and chemosensory proteins in Grapholita molesta. Zhao, Zhiguo,Liu, Baoling,Rong, Erhua,Zhang, Lijun,Guo, Yanqiong,Ma, Ruiyan,Li, Jie,Kong, Weina.

[19]Cloning, Expression, and Characterization of a Milk-Clotting Aspartic Protease Gene (Po-Asp) from Pleurotus ostreatus. Yin, Chaomin,Ma, Aimin,Zheng, Liesheng,Chen, Liguo,Tan, Qi,Shang, Xiaodong.

[20]Cloning and Expression of Two Soluble Acid Invertase Gene Isoforms from Rhododendron. He Lisi,Su Jiale,Liu Xiaoqing,Li Chang,Chen Shangping. 2014

作者其他论文 更多>>

微信小程序