Improved taxol production in Nodulisporium sylviforme derived from inactivated protoplast fusion

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Ma, Xi;Li, Xiuliang;Wang, Xin;Ping, Wenxiang;Zhou, Dongpo;Zhao, Kai;Sun, Lixin

作者机构：

关键词： Taxol;endophytic fungi;protoplast preparation;protoplast fusion

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 20 期

页码：

收录情况： SCI

摘要： Inactivated protoplast fusion by UV irradiation and UV+LiCl mutation was conducted using Nodulisporium sylviforme strain UV40-19 and UL50-6 to breed a high taxol-producing fungus. Qualitative and quantitative analysis of taxol production was confirmed using thin-layer chromatography, high performance liquid chromatography and mass spectrometry. The protoplasts of UV40-19 and UL50-6 were fully inactivated by heating at 54 degrees C for 5 min and by UV irradiation (30 w UV light and vertical distance 30 cm) for 85 s. The highest fusion rate (14.31 +/- 1.13%) between UV40-19 and UL50-6 was obtained under the conditions of 35% PEG, 90 s fusion time and the addition of 0.01 mol/l CaCl2. One high taxol production strain HDF-68 was obtained. The taxol production was up to 468.62 +/- 37.49 mu g/l, which was increased by 24.51 and 19.35% compared with the parental strain UV40-19 and UL50-6, respectively. This study provided a good basis for the application of this technique to the breeding of the strains with high taxol output.

分类号：

  • 相关文献

[1]Production of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool. Hu, Q,Andersen, SB,Dixelius, C,Hansen, LN. 2002

[2]Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana. Sheng, Xiaoguang,Liu, Fan,Zhu, Yuelin,Zhao, Hong,Zhang, Li,Chen, Bin.

[3]Comparative proteomic analyses of two Taxus species (Taxus X media and Taxus mairei) reveals variations in the metabolisms associated with paclitaxel and other metabolites. Juan Hao,Shen, Chenjia,Hong Guo,Xinai Shi,Ye Wang,Qinghua Wan,Yao-Bin Song,Lei Zhang,Ming Dong,Chenjia Shen. 2017

[4]Identification of potential genes that contributed to the variation in the taxoid contents between two Taxus species (Taxus media and Taxus mairei). Yu, Chunna,Guo, Hong,Zhang, Yangyang,Song, Yaobin,Pi, Erxu,Dong, Ming,Wang, Huizhong,Shen, Chenjia,Yu, Chunna,Guo, Hong,Wang, Huizhong,Shen, Chenjia,Song, Yaobin,Dong, Ming,Yu, Chenliang,Zhang, Lei,Zheng, Bingsong. 2017

[5]Investigation of fermentation conditions and optimization of medium for taxol production from taxol-producing fungi. Zhao, Kai,Li, Zhugang,Ge, Nan,Li, Xiuliang,Wang, Xin,Zhou, Dongpo,Zhao, Kai. 2011

[6]Diversity of Endophytic Fungi from Different Verticillium-Wilt-Resistant Gossypium hirsutum and Evaluation of Antifungal Activity Against Verticillium dahliae In Vitro. Li, Zhi-Fang,Wang, Ling-Fei,Feng, Zi-Li,Zhao, Li-Hong,Shi, Yong-Qiang,Zhu, He-Qin.

[7]Diversity and biocontrol potential of endophytic fungi in Brassica napus. Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Qinghua,Zhang, Jing,Yang, Long,Jiang, Daohong,Li, Guoqing,Zhang, Lei,Chen, Weidong. 2014

[8]Mycosphere Essays 14: Assessing the aggressiveness of plant pathogenic Botryosphaeriaceae. Manawasinghe, I. S.,Chethana, K. W. T.,Zhang, W.,Yan, J. Y.,Li, X. H.,Manawasinghe, I. S.,Hyde, K. D.,Chethana, K. W. T.,Phillips, A. J. L.,Zhang, W.,Zhao, W. S.. 2016

[9]Biocontrol potential of endophytic fungi in medicinal plants from Wuhan Botanical Garden in China. Xiang, Libo,Gong, Shuangjun,Yang, Lijun,Xue, MinFeng,Zeng, FanSong,Zhang, XueJiang,Shi, WenQi,Wang, Hua,Yu, Dazhao,Hao, Jianjun.

[10]Antioxidant activity of secondary metabolites and mycelium extracts of endophytic fungi isolated from Astragalus monadelphus. Wang, Y. G.,Yang, G. R.,Yang, M. J.,Li, J.,Liu, X. F.,Wang, M. G.,Wang, F.,Wang, X. L.. 2016

[11]Isolation an antimicrobial action of endophytic fungi from sophora flavescens and effects on microorganism circumstances in soil. He, Lu,Wang, Yong,Xu, Hong Bo,Yu, Na,Liu, Na. 2013

[12]In vitro antioxidant activities of endophytic fungi isolated from the liverwort Scapania verrucosa. Zeng, P. Y.,Wu, J. G.,Liao, L. M.,Wu, J. Z.,Chen, T. -Q.,Wong, K. -H.. 2011

[13]Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus. Ren, Na,Liu, Jiajia,Yang, Dongliang,Hong, Juan,Chen, Jianhua,Luan, Mingbao. 2012

[14]Antimicrobial action of an endophytic fungi from Sophor flavescens and structure identification of its active constituent. Yu, Na,He, Lu,Wang, Yong,Xu, Hongbo,Liu, Dandan,Liu, Na.

[15]Screening of endophytic fungi that promote the growth of Euphorbia pekinensis. Dai, Chuan-chao,Yu, Bo-yang,Li, Xia. 2008

[16]Extensive host range of an endophytic fungus affects the growth and physiological functions in rice (Oryza sativa L.). Yuan, Zhi-Lin,Dai, Chuan-Chao,Li, Xia,Tian, Lin-Shuang,Wang, Xing-Xiang. 2007

[17]Endophytic fungal diversity and space-time dynamics in sugar beet. Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai,Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai.

[18]Phylogenetic analysis of Plectosphaerella species based on multi-locus DNA sequences and description of P. sinensis sp nov.. Su, Lei,Deng, Hui,Niu, Yong-Chun.

[19]Secondary metabolites from the endophytic fungus Pestalotiopsis virgatula isolated from the mangrove plant Sonneratia caseolaris. Roensberg, David,Debbab, Abdessamad,Proksch, Peter,Aly, Arnal H.,Mandi, Attila,Kurtan, Tibor,Wray, Victor,Dai, Haofu.

作者其他论文 更多>>

微信小程序