Improved taxol production in Nodulisporium sylviforme derived from inactivated protoplast fusion

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Ma, Xi;Li, Xiuliang;Wang, Xin;Ping, Wenxiang;Zhou, Dongpo;Zhao, Kai;Sun, Lixin

作者机构：

关键词： Taxol;endophytic fungi;protoplast preparation;protoplast fusion

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 20 期

页码：

收录情况： SCI

摘要： Inactivated protoplast fusion by UV irradiation and UV+LiCl mutation was conducted using Nodulisporium sylviforme strain UV40-19 and UL50-6 to breed a high taxol-producing fungus. Qualitative and quantitative analysis of taxol production was confirmed using thin-layer chromatography, high performance liquid chromatography and mass spectrometry. The protoplasts of UV40-19 and UL50-6 were fully inactivated by heating at 54 degrees C for 5 min and by UV irradiation (30 w UV light and vertical distance 30 cm) for 85 s. The highest fusion rate (14.31 +/- 1.13%) between UV40-19 and UL50-6 was obtained under the conditions of 35% PEG, 90 s fusion time and the addition of 0.01 mol/l CaCl2. One high taxol production strain HDF-68 was obtained. The taxol production was up to 468.62 +/- 37.49 mu g/l, which was increased by 24.51 and 19.35% compared with the parental strain UV40-19 and UL50-6, respectively. This study provided a good basis for the application of this technique to the breeding of the strains with high taxol output.

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