Genome-editing of a circadian clock gene TaPRR95 facilitates wheat peduncle growth and heading date

文献类型： 外文期刊

第一作者： Fu, Mingxue

作者： Fu, Mingxue;Liu, Shaoshuai;Che, Yuqing;Cui, Dada;Deng, Zhongyin;Li, Yang;Zou, Xinyu;Geng, Shuaifeng;Li, Aili;Mao, Long;Fu, Mingxue;Liu, Shaoshuai;Che, Yuqing;Cui, Dada;Deng, Zhongyin;Li, Yang;Zou, Xinyu;Geng, Shuaifeng;Li, Aili;Mao, Long;Kong, Xingchen;Chen, Guoliang;Zhang, Min;Liu, Yifan;Wang, Xiang;Liu, Wei;Liu, Danmei

作者机构：

关键词： Pseudo-response regulator (PRR); TaPRR95; CRISPR/Cas9; Plant height; Heading date; Photosynthesis; Phytohormone; Wheat

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：7.1； 五年影响因子：6.4 ）

ISSN： 1673-8527

年卷期： 2024 年 51 卷 10 期

页码：

收录情况： SCI

摘要： Plant height and heading date are important agronomic traits in wheat (Triticum aestivum L.) that affect final grain yield. In wheat, knowledge of pseudo-response regulator (PRR) genes on agronomic traits is limited. Here, we identify a wheat TaPRR95 gene by genome-wide association studies to be associated with plant height. Triple allele mutant plants produced by CRISPR/Cas9 show increased plant height, particularly the peduncle, with an earlier heading date. The longer peduncle is mainly caused by the increased cell elongation at its upper section, whilst the early heading date is accompanied by elevated expression of flowering genes, such as TaFT and TaCO1. A peduncle-specific transcriptome analysis reveals up-regulated photosynthesis genes and down-regulated IAA/Aux genes for auxin signaling in prr95 aabbdd plants that may act as a regulatory mechanism to promote robust plant growth. A haplotype analysis identifies a TaPRR95B haplotype (Hap2) to be closely associated with reduced plant height and increased thousand-grain weight. Moreover, the Hap2 frequency is higher in cultivars than that in landraces, suggesting the artificial selection on the allele during wheat breeding. These findings suggest that TaPRR95 is a regulator for plant height and heading date, thereby providing an important target for wheat yield improvement.

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