The GhEB1C gene mediates resistance of cotton to Verticillium wilt

文献类型： 外文期刊

第一作者： Xu, Jianglin

作者： Xu, Jianglin;Zhou, Ting;Wang, Peilin;Wang, YongQiang;Yang, Yejun;Pu, Yuanchun;Sun, Guoqing;Xu, Jianglin;Wang, YongQiang;Chen, Quanjia;Zhou, Ting;Yang, Yejun;Wang, Peilin;Pu, Yuanchun

作者机构：

关键词： End-binding protein 1C (EB1C) family; GhEB1C; Gossypium hirsutum; Microtubule-associated proteins; Plant hormones

期刊名称：PLANTA （ 影响因子：3.8； 五年影响因子：4.4 ）

ISSN： 0032-0935

年卷期： 2024 年 260 卷 5 期

页码：

收录情况： SCI

摘要： Cotton, as a crucial cash and oil crop, faces a significant threat from Verticillium wilt, a soil-borne disease induced by Verticillium dahliae, severely impacting cotton growth and development. Investigating genes associated with resistance to Verticillium wilt is paramount. We identified and performed a phylogenetic analysis on members of the EB1 family associated with Verticillium wilt in this work. GhEB1C was discovered by transcriptome screening and was studied for its function in cotton defense against V. dahliae. The RT-qPCR analysis revealed significant expression of the GhEB1C gene in cotton leaves. Subsequent localization analysis using transient expression demonstrated cytoplasmic localization of GhEB1C. VIGS experiments indicated that silencing of the GhEB1C gene significantly increased susceptibility of cotton to V. dahliae. Comparative RNA-seq analysis showed that GhEB1C silenced plants exhibited altered microtubule-associated protein pathways and flavonogen-associated pathways, suggesting a role for GhEB1C in defense mechanisms. Overexpression of tobacco resulted in enhanced resistance to V. dahliae as compared to wild-type plants. Furthermore, our investigation into the relationship between the GhEB1C gene and plant disease resistance hormones salicylic axid (SA) and jasmonic acid (JA) revealed the involvement of GhEB1C in the regulation of the SA pathway. In conclusion, our findings demonstrate that GhEB1C plays a crucial role in conferring immunity to cotton against Verticillium wilt, providing valuable insights for further research on plant adaptability to pathogen invasion.

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