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PRRSV XH-GD株NSP9缺失感染性克隆的构建

文献类型: 中文期刊

作者: 赵孟孟 1 ; 冯丽丽 2 ; 冯嘉萍 2 ; 冯松林 2 ; 王文佳 2 ; 张桂红 2 ;

作者机构: 1.河南农业大学牧医工程学院

2.河南农业大学牧医工程学院;广东省动物源性人兽共患病预防与控制重点实验室国家生猪种业工程技术研究中心/华南农业大学兽医学院;河南省农业科学院农业经济与信息研究所;河南牧业经济学院制药工程学院

关键词: PRRSV;NSP9;缺失构建;复制;感染性克隆;拯救

期刊名称: 黑龙江畜牧兽医

ISSN: 1004-7034

年卷期: 2016 年 23 期

页码: 10-13+295

收录情况: 北大核心

摘要: PRRSV的非结构蛋白NSP9是病毒的一种高度保守的非结构蛋白,为了研究病毒NSP9基因是否为病毒复制所必需基因,将PRRSV XH-GD株的NSP9基因通过融合PCR方法进行缺失,将缺失NSP9基因的感染性克隆转染BHK细胞并进行病毒的拯救。结果表明:不能进行有效的拯救,对照组可以成功拯救。说明NSP9基因的缺失对病毒产生致死的影响,而不是操作手段的问题导致无法成功拯救。

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