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几种提纯RHDV方法的比较研究

文献类型: 中文期刊

作者: 杨龙圣 1 ; 薛家宾 2 ; 丁国明 3 ; 徐为中 2 ; 陈兴祥 2 ; 孔祥峰 1 ; 胡元亮 1 ;

作者机构: 1.南京农业大学动物医学院

2.江苏省农业科学院兽医研究所

3.江苏省通州市新联镇农业服务中心

关键词: RHDV;凝胶过滤层析法;葡聚糖-200;离子交换层析法;ELISA

期刊名称: 西北农业学报

ISSN: 1004-1389

年卷期: 2006 年 15 卷 02 期

页码: 13-16+41

收录情况: 北大核心 ; CSCD

摘要: 运用离子交换层析法、凝胶过滤层析法和密度梯度离心法对已作初步处理的RHDV分别进行提纯,并通过血凝性鉴定、抗原蛋白含量检测、ELISA和SDS-PAGE凝胶电泳等方法检测其纯度。结果表明,通过Sephadex-200凝胶过滤层析法所提纯病毒的血凝性和蛋白浓度最高;稀释至相同的蛋白浓度之后,该法所提纯的病毒ELISA检测的OD值最大;SDS-PAGE电泳条带1条,经免疫转印分析证明,该条带为RHDV蛋白特异性条带。表明通过该法提纯的病毒,回收率最高、蛋白最纯。

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