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Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54

文献类型： 外文期刊

作者： Wei, Jianhui 1 ; Liu, Chuancheng 1 ; He, Xinyan 1 ; Abbas, Bilal 1 ; Chen, Qi 1 ; Li, Zhaolong 2 ; Feng, Zhihua 1 ;

作者机构： 1.Fujian Normal Univ Qishan Campus, Coll Life Sci, Biomed Res Ctr South China, Fujian Key Lab Innate Immune Biol, Fuzhou 350117, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350117, Peoples R China

关键词： African swine fever virus; pseudorabies virus; CD2v; p54; vaccine

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) leads to high mortality in domestic pigs and wild boar, and it is caused by the African swine fever virus (ASFV). Currently, no commercially available vaccine exists for its prevention in China. In this study, we engineered a pseudorabies recombinant virus (PRV) expressing ASFV CD2v and p54 proteins (PRV-triangle TK-(CD2v)-triangle gE-(p54)) using CRISPR/Cas9 and homologous recombination technology. PRV-triangle TK-(CD2v)-triangle gE-(p54) effectively delivers CD2v and p54, and it exhibits reduced virulence. Immunization with PRV-triangle TK-(CD2v)-triangle gE-(p54) neither induces pruritus nor causes systemic infection and inflammation. Furthermore, a double knockout of the TK and gE genes eliminates the depletion of T, B, and monocytes/macrophages in the blood caused by wild-type viral infection, decreases the proliferation of granulocytes to eliminate T-cell immunosuppression from granulocytes, and enhances the ability of the immune system against PRV infection. An overexpression of CD2v and p54 proteins does not alter the characteristics of PRV-triangle TK/triangle gE. Moreover, PRV-triangle TK-(CD2v)-triangle gE-(p54) successfully induces antibody production via intramuscular (IM) vaccination and confers effective protection for vaccinated mice upon challenge. Thus, PRV-triangle TK-(CD2v)-triangle gE-(p54) demonstrates good immunogenicity and safety, providing highly effective protection against PRV and ASFV. It potentially represents a suitable candidate for the development of a bivalent vaccine against both PRV and ASFV infections.

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