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cDNA cloning and mRNA expression of the translationally controlled tumor protein (TCTP) gene from Japanese sea perch (Lateolabrax japonicus)

文献类型: 外文期刊

作者: Qiu, LH 1 ; Song, LS 2 ; Wu, LT 2 ; Xu, W 2 ; Jiang, SG 2 ;

作者机构: 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

关键词: translationally controlled tumor protein;lateolabrax japonicus;cDNA cloning;mRNA expression

期刊名称:ACTA OCEANOLOGICA SINICA ( 影响因子:1.431; 五年影响因子:1.445 )

ISSN:

年卷期:

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收录情况: SCI

摘要: A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of ze-brafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosyla-tion site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver.

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