您好,欢迎访问中国水产科学研究院 机构知识库!

Cloning, characterization and expression analysis of interleukin-10 from the zebrarish (Danio rerion)

文献类型: 外文期刊

作者: Zhang, DC 1 ; Shao, YQ 2 ; Huang, YQ 2 ; Jiang, SG 2 ;

作者机构: 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Aquaculture & Biotechnol Div, Guangzhou 510300, Peoples R China

2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Aquaculture & Biotechnol Div, Guangzhou 510300, Peoples R China; Shanghai Fisheries Univ, Shanghai 200090, Peoples R China

关键词: cytokine;genomic analysis;interleukin;zebrafish

期刊名称:JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY ( 影响因子:2.021; 五年影响因子:2.1 )

ISSN: 1225-8687

年卷期: 2005 年 38 卷 5 期

页码:

收录情况: SCI

摘要: Cytokines are proteins produced by many different cells of the immune system and play a significant role in initiating and regulating the inflammatory process. In this research, an important cytokine, interieukin-10 (IL-10) gene, has been identified and characterized from zebrafish (Danio rerio) genome database. Zebrafish IL-10 is located within a 2690 lip fragment and contains five exons and four introns, sharing the same organization with mammalian IL-10 genes. An open reading frame of 543 lip was found to encode a putative 180 amino acid protein with a signal peptide of 22 amino acids, which shares 29.7-80.9% homology with amino acid sequences of other known IL10. The signature motif of IL-10 is also conserved in zebrafish IL-10. The predicted transcript was finally confirmed by sequencing of cDNA clones. Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the tissue distribution and expression regulation of this gene in seven organs of normal and lipopolysaccharide (LPS) stimulation zebrafish. The results demonstrated that this gene was expressed slightly in normal kidney, gill and gut, no expression was detected in other four tissues. The expression was clearly upregulated after LPS stimulation. Using the ideal zebrafish model, further study of IL-10 characterization and function may provide insight on the understanding of the innate immune system.

  • 相关文献

[1]The physiological performance and immune response of juvenile turbot (Scophthalmus maximus) to nitrite exposure. Jia, Rui,Lei, Ji-Lin,Han, Cen.

[2]Effects of curcumin on antioxidative activities and cytokine production in Jian carp (Cyprinus carpio var. Jian) with CCl4-induced liver damage. Cao, Liping,Ding, Weidong,Du, Jingliang,Jia, Rui,Liu, Yingjuan,Zhao, Caiyuan,Shen, Yujin,Yin, Guojun,Cao, Liping,Ding, Weidong,Du, Jingliang,Jia, Rui,Liu, Yingjuan,Zhao, Caiyuan,Shen, Yujin,Yin, Guojun.

[3]The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish. Mu, Xiyan,Shen, Gongming,Huang, Ying,Luo, Jianbo,Li, Yingren,Zhu, Lizhen,Wang, Chengju,Li, Xuefeng,Qi, Suzhen.

[4]Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish. Yin, Guojun,Kitazawa, Takio,Teraoka, Hiroki,Yin, Guojun,Cao, Liping,Du, Jinliang,Jia, Rui,Kubota, Akira.

[5]Characterization and toxicology evaluation of chitosan nanoparticles on the embryonic development of zebrafish, Danio rerio. Wang, Yanbo,Zhou, Jinru,Liu, Lin,Fu, Linglin,Huang, Changjiang,Zhou, Deqing.

[6]Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio). Tian, Juan,He, Gen,Mai, Kangsen,Liu, Chengdong,Tian, Juan.

[7]Assessment of Sediment Quality of Yangtze River Estuary Using Zebrafish (Danio rerio) Embryos. Wu, Lingling,Chen, Ling,Zhang, Yalei,Zhao, Jianfu,Gao, Hongwen,Wu, Lingling,Hou, Junli.

[8]Derivation of a pluripotent embryonic cell line from red sea bream blastulas. Chen, SL,Ye, HQ,Sha, ZX,Hong, Y.

[9]Bioinformatic Analyses of CCCH-Zinc Finger Family in Zebrafish (Danio rerio). Shui, Yan,Xu, Zeng-Hong,Zhou, Xin,Zhou, Xin. 2014

[10]CS5931, a Novel Polypeptide in Ciona savignyi, Represses Angiogenesis via Inhibiting Vascular Endothelial Growth Factor (VEGF) and Matrix Metalloproteinases (MMPs). Liu, Ge,Wu, Ning,Liu, Ge,Liu, Ming,Wei, Jianteng,Huang, Haijuan,Zhang, Yuyan,Zhao, Jin,Xiao, Lin,Zheng, Lanhong,Lin, Xiukun. 2014

[11]Proteomic profile and toxicity pathway analysis in zebrafish embryos exposed to bisphenol A and di-n-butyl phthalate at environmentally relevant levels. Dong, Xing,Qiu, Xuchun,Xu, Hai,Wu, Xiangyang,Qiu, Xuchun,Meng, Shunlong,Yang, Ming. 2018

[12]Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics. Liu, Guangjin,Zhang, Wei,Liu, Yongjie,Yao, Huochun,Lu, Chengping,Xu, Pao. 2014

[13]Soybean saponin modulates nutrient sensing pathways and metabolism in zebrafish. Tian, Juan,Wang, Kaidi,Wang, Xuan,Zhou, Huihui,Liu, Chengdong,Mai, Kangsen,He, Gen,Tian, Juan,Wen, Hua,He, Gen. 2018

[14]Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos. Tanaka, Yasuaki,Fujiwara, Mari,Shindo, Asako,Kitazawa, Takio,Teraoka, Hiroki,Shindo, Asako,Yin, Guojun. 2018

[15]The developmental effect of difenoconazole on zebrafish embryos: A mechanism research. Mu, Xiyan,Chai, Tingting,Zhu, Lizhen,Li, Xuefeng,Wang, Chengju,Mu, Xiyan,Huang, Ying,Shen, Gongming,Li, Yingren,Wang, Kai.

[16]Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity. Xu, Hongyan,Li, Caixia,Li, Yan,Ng, Grace Hwee Boon,Liu, Chunsheng,Zhang, Xiaoyan,Gong, Zhiyuan,Xu, Hongyan,Liu, Chunsheng.

[17]Molecular cloning and characterization of the Myf5 gene in sea perch (Lateolabrax japonicus). Ye, Han-Qing,Chen, Song-Lin,Xu, Jian-Yong.

[18]Development of a positive-negative selection procedure for gene targeting in fish cells. Chen, SL,Hong, YH,Schartl, M.

作者其他论文 更多>>