文献类型: 外文期刊
作者: Zhang, XY 1 ; Yang, JQ 2 ; Zhang, DC 2 ; Deng, FJ 2 ; Yu, LN 2 ; Fang, YL 2 ;
作者机构: 1.Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China
2.Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China; Chinese Acad Fishery Sci, Yangtze River Fisheries Inst, Jingsha 43400, Peoples R China
关键词: fish genome;RAPD analysis;genetic diversity;genomic;DNA;extract
期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )
ISSN: 1000-3282
年卷期: 1999 年 26 卷 5 期
页码:
收录情况: SCI
摘要: Total 40 random primers of OPN and OPM groups made in Operon Co. were applied to the RAPD analysis of H. molitrix and A. nobilis. Besides establishment of species-specific RAPD electrophoretogram, some kinds of primers which can produce molecular genetic markers of RAPD with individuality- or population-specificity of these fish were found. A modified method to extracting genomic DNA from peripheral blood cells of fish, and the application prospects of RAPD technique in genetic diversity and variation of fish, and in fishery management and the evaluation of fish resources were discussed.
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