Identification and Characterization of a New Type of Holin-Endolysin Lysis Cassette in Acidovorax oryzae Phage AP1
文献类型： 外文期刊
作者： Zhang, Muchen 1 ; Wang, Yanli 2 ; Chen, Jie 1 ; Hong, Xianxian 1 ; Xu, Xinyan 1 ; Wu, Zhifeng 1 ; Ahmed, Temoor 1 ; Loh, Belinda 3 ; Leptihn, Sebastian 3 ; Hassan, Sabry 4 ; Hassan, Mohamed M. 4 ; Sun, Guochang 2 ; Li, Bin 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, State Key Lab Rice Biol & Minist Agr, Hangzhou 310058, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
3.Zhejiang Univ, Univ Edinburgh Inst, Hangzhou 314400, Peoples R China
4.Taif Univ, Coll Sci, Dept Biol, At Taif 21944, Saudi Arabia
关键词： Acidovorax oryzae phage AP1; lysis cassette; holin; endolysin
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 2 期
页码：
收录情况： SCI
摘要： Phages utilize lysis systems to allow the release of newly assembled viral particles that kill the bacterial host. This is also the case for phage AP1, which infects the rice pathogen Acidovorax oryzae. However, how lysis occurs on a molecular level is currently unknown. We performed in silico bioinformatics analyses, which indicated that the lysis cassette contains a holin (HolAP) and endolysin (LysAP), which are encoded by two adjacent genes. Recombinant expression of LysAP caused Escherichia coli lysis, while HolAP arrested growth. Co-expression of both proteins resulted in enhanced lysis activity compared to the individual proteins alone. Interestingly, LysAP contains a C-terminal region transmembrane domain, which is different from most known endolysins where a N-terminal hydrophobic region is found, with the potential to insert into the membrane. We show that the C-terminal transmembrane domain is crucial for protein localization and bacterial lysis in phage AP1. Our study characterizes the new phage lysis cassette and the mechanism to induce cell disruption, giving new insight in the understanding of phage life cycles.
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