Appropriate leucine supplementation promotes glucose metabolism and enhances energy homeostasis in juvenile crucian carp (Carassius auratus gibelio var. CAS III)
文献类型： 外文期刊
作者： Miao, Linghong 1 ; Zhang, Yuanyuan 3 ; Lin, Yan 1 ; Liu, Bo 1 ; Ge, Xianping 1 ;
作者机构： 1.Chinese Acad Fishery Sci CAFS, Key Lab Genet Breeding Aquat Anim & Aquaculture B, Freshwater Fisheries Res Ctr FFRC, Wuxi 214081, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
3.Shandong Freshwater Fisheries Res Inst, 162 Jiefang Rd, Jinan 250013, Peoples R China
4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, 9 East Shanshui Rd, Wuxi 214081, Jiangsu, Peoples R China
关键词： Leucine; High-carbohydrate metabolism; Energy homeostasis; Transcriptome; Carassius auratus gibelio var; CAS III
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）
ISSN： 1744-117X
年卷期： 2021 年 40 卷
页码：
收录情况： SCI
摘要： In order to characterize the molecular mechanisms by which leucine regulates carbohydrate metabolism and energy homeostasis, juvenile crucian carps (Carassius auratus gibelio var. CAS III) fed with a high carbohydrate diet were supplemented with different levels of dietary leucine: 0% (Leu0), 0.4% (Leu4), 0.8% (Leu8), 1.2% (Leu12), 1.6% (Leu16), 2.0% (Leu20), and 5.0% (Leu50). After 8 weeks, RNA sequencing was performed on samples collected from the Leu0, Leu8, Leu12 and Leu50 groups. Differentially expressed genes were then detected and analyzed. The results showed a total of 91.6 Gb of clean bases were generated. Moreover, a total of 1131, 5254, and 1539 DEGs were detected in Leu8, Leu12, and Leu50 compared with Leu0, respectively, encompassing 161 common DEGs. STEM analysis elucidated four significant clusters of DEGs that were associ-ated with "glycerophospholipid metabolism," "glycerolipid metabolism," "PPAR signaling pathway," and "adi-pocytokine signaling pathway." Moreover, the mRNA expression levels of acyl-CoA synthetase long chain family member 5 (ACSL5), choline kinase beta (CHKB), cryptochrome-1 (CRY1), lon protease homolog 2, peroxisomal isoform X2 (LONP2), lipin 1 (LPIN1), membrane bound O-acyltransferase domain containing 2 (MBOAT2), phosphoenolpyruvate carboxykinase 1 (PEPCK), and uridine-cytidine kinase 2b (UCK2b) were then further investigated in all leucine treatment groups at starvation times of 0 h, 24 h, and 48 h. The results revealed that the expression levels of UCK2b and MBOAT2 were negatively correlated with the addition of leucine, whereas CHKB, LONP2, CRY1, PEPCK, and LPIN1 were positively correlated. In conclusion, dietary leucine supplemen-tation below 1.2% enhanced carbohydrate metabolism in juvenile crucian carp fed with a high-carbohydrate diet, whereas concentrations above 2.0% is a better choice for energy homeostasis under starvation.
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