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Combinations but Not a Single PlpE Epitope Induces Host Protective Immunity against Pasteurella multocida

文献类型： 外文期刊

作者： Zhu, Weifeng 1 ; Zhou, Banghui 1 ; Chen, Lu 3 ; Zhao, Juan 2 ; Rao, Huaqin 4 ;

作者机构： 1.Hebei Agr Univ, Coll Vet Med, Baoding, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Peoples R China

3.Tibetan Agr & Anim Husb Coll, Coll Anim Sci, Linzhi, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China

关键词： epitope; immunoprotection; Pasteurella multocida; PlpE; synergism

期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.1； 五年影响因子：3.2 ）

ISSN： 0019-9567

年卷期： 2023 年 91 卷 3 期

页码：

收录情况： SCI

摘要： Pasteurella multocida is the causative agent of a wide range of diseases (pasteurellosis) and a zoonotic pathogen in humans. Recombinant subunit vaccines are hot spots in recent pasteurellosis vaccine development. A chimeric vaccine is also constructed for rabbit hemorrhagic disease virus (RHDV) protective antigen VP60 chimeric with fragments of Pasteurella multocida protective antigen PlpE. The protective efficacy of the chimeric vaccine against P. multocida is not as high as that of PlpE, and the reason is not well known. In this study, we analyzed the linear B-cell epitopes of PlpE and then assessed the protective efficacy of these epitopes and their combinations. It was found that the immunodominant region of PlpE was mainly located in the region between the 21st to the 185th amino acids from the N terminus. Overlapping peptide scanning results demonstrated that this region contained six nonoverlapping epitopes, and epitope E was the predominant epitope. Chimeric protein antigens were constructed of single nonoverlapping PlpE epitopes or their combinations chimeric with the RHDV VP60 P domain. Immunization with recombinant antigen chimeric with a single PlpE epitope exhibited poor immunoprotection, whereas immunization with recombinant antigen chimeric with PlpE epitope combinations (epitopes A and E; epitopes C and E; epitopes A, C, and E; and epitopes B, D, and F) exhibited significant immunoprotection. In a word, P. multocida protective antigen PlpE contained six nonoverlapping linear B-cell epitopes, and combinations but not a single epitope induced host protective immunity. Our work will give help for future chimeric vaccine design. Pasteurella multocida is the causative agent of a wide range of diseases (pasteurellosis) and a zoonotic pathogen in humans. Recombinant subunit vaccines are hot spots in recent pasteurellosis vaccine development.

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