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Rabbit Hemorrhagic Disease Virus Non-structural Protein 6 Induces Apoptosis in Rabbit Kidney Cells

文献类型： 外文期刊

作者： Chen, Mengmeng 1 ; Liu, Xing 1 ; Hu, Bo 1 ; Fan, Zhiyu 1 ; Song, Yanhua 1 ; Wei, Houjun 1 ; Qiu, Rulong 1 ; Xu, Weizhong; 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med, Key Lab Vet Biol Engn & Technol,Minist Agr, Nanjing, Jiangsu, Peoples R China

2.Ji

关键词： rabbit hemorrhagic disease virus; non-structural protein 6; 3C-like protease; apoptosis; rabbit hemorrhagic disease

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： Rabbit hemorrhagic disease (RHD) is a highly contagious disease caused by rabbit hemorrhagic disease virus (RHDV). Previous research has shown that RHDV induces apoptosis in numerous cell types, although the molecular mechanisms underlying the apoptosis induced by RHDV are not well understood. One possible factor is non-structural protein 6 (NSP6), a 3C-like protease that plays an important role in processing viral polyprotein precursors into mature non-structural proteins. To fully establish a role for NSP6, the present study examined the effects of ectopic expression of the protein in rabbit (RK13) and human (HeLa and HepG2) cells. We found that NSP6 suppressed cell viability and promoted apoptosis in all three cell types in a dose-dependent manner. We also identified increased caspase-3, -8, and -9 activities in RK13 cell, and an increased Bax to Bcl2 mRNA ratio. Mechanistically, the ability of NSP6 to induce apoptosis was impaired by mutation of the catalytic His27 residue. Our study has shown that RHDV NSP6 can induce apoptosis in host cells and is likely an important contributor to RHDV-induced apoptosis and pathogenesis.

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