RPA-Cas12aDS: A visual and fast molecular diagnostics platform based on RPA-CRISPR-Cas12a method for infectious bursal disease virus detection
文献类型： 外文期刊
作者： Zheng, Su-ya 1 ; Ma, Li-li 2 ; Wang, Xiao-li 2 ; Lu, Li-xin 2 ; Ma, Sun-ting 2 ; Xu, Bin 2 ; Ouyang, Wei 1 ;
作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
2.Minist Agr, Inst Vet Med, Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
3.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
5.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
关键词： CRISPR-Cas12a system; RPA assay; RPA-CRISPR-Cas12a; Detection; Cleavage
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.623； 五年影响因子：2.239 ）
ISSN： 0166-0934
年卷期： 2022 年 304 卷
页码：
收录情况： SCI
摘要： Infectious bursal disease (IBD), a major disease of birds, is caused by infectious bursal disease virus (IBDV). The disease can lead to immunosuppression, resulting in huge economic losses in the poultry industry. A specific, rapid, and simple detection method is important for the early diagnosis and prevention and control of IBDV. In this study, we established a naked-eye visual IBDV detection method, named "RPA-Cas12aDS", by combining recombinase polymerase amplification (RPA) with CRISPR-Cas12a-based nucleic acid detection. The detection process can be accomplished in 50 min, and uncapping contamination can be avoided. The detection results can be observed under blue or UV light. We used the RPA-Cas12aDS method to detect IBDV in bursa of Fabricius tissue samples of chickens, and the results were consistent with those obtained using commercial RT-PCR kits. This method presents great potential for visual, rapid, and point-of-care molecular diagnostics of IBDV in poultry.
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