Antifungal compounds from Bacillus subtilis B-FS06 inhibiting the growth of Aspergillus flavus
文献类型： 外文期刊
作者： Zhang, Ting 1 ; Shi, Zhi-Qi 1 ; Hu, Liang-Bin 3 ; Cheng, Luo-Gen 4 ; Wang, Fei 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Nanjing 210014, Peoples R China
2.Jiangxi Acad Forestry, Jiangxi Provicial Key Lab Plant Biotechnol, Nanchang 330032, Peoples R China
3.Henan Inst Sci & Technol, Sch Food, Xinxiang 453003, Peoples R China
4.Nanjing Normal Univ, Coll Life Sci, Nanjing 210097, Peoples R China
关键词： Aspergillus flavus;Bacillus subtilis
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）
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收录情况： SCI
摘要： The cell-free culture filtrate (CCF) was prepared from a culture of an Aspergillus flavus antagonist, Bacillus subtilis B-FS06. The CCF inhibited the growth and spore germination of A. flavus at a series of concentrations (10, 25, 50%) (v/v). It still retained the activity after treatment at pH values ranging from 2 to 12 for 24 h or at 100 pC for 30 min. The antifungal activity, however, was reduced by 30% after treatment at 121 pC for 20 min. After purification by anion exchange chromatography, gel filtration chromatography and HPLC, the active compounds revealed six ion peaks: [M-H] m/z = 1006.78, 1020.71, 1034.74, 1049.54, 1056.78, and 1071.64 by using electrospray ionization mass spectrometry (ESI-MS) analysis. In the presence of the active compounds at 200 og/g, the growth of A. flavus on peanuts was completely inhibited.
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