Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension
文献类型： 外文期刊
作者： Zhuan, Qingrui 1 ; Li, Jun 2 ; Du, Xingzhu 1 ; Zhang, Luyao 3 ; Meng, Lin 3 ; Luo, Yuwen 1 ; Zhou, Dan 1 ; Liu, Hongyu 1 ; Wan, Pengcheng 4 ; Hou, Yunpeng 3 ; Fu, Xiangwei 1 ;
作者机构： 1.China Agr Univ, Natl Engn Lab Anim Breeding, Minist Agr & Rural Affairs,Key Lab Anim Genet Bre, Key Lab Anim Genet Improvement,Coll Anim Sci & Te, Beijing, Peoples R China
2.Hebei Med Univ, Hosp 1, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang, Hebei, Peoples R China
3.China Agr Univ, Coll Biol Sci, State Key Labs Agrobiotechnol, Beijing, Peoples R China
4.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Bree, Shihhotze, Peoples R China
关键词： Cortical tension; Meiosis; Mitochondria; Oocytes; Procyanidin B2; Vitrification
期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.175； 五年影响因子：6.853 ）
ISSN： 1674-9782
年卷期： 2022 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Background Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility preservation. Antioxidants were always used to antagonist the oxidative stress caused by vitrification. However, the comprehensive mechanism underlying the protective role of antioxidants has not been studied. Procyanidin B2 (PCB2) is a potent natural antioxidant and its functions in response to vitrification are still unknown. In this study, the effects of PCB2 on vitrified-thawed oocytes and subsequent embryo development were explored, and the mechanisms underlying the protective role of PCB2 were systematically elucidated. Results Vitrification induced a marked decline in oocyte quality, while PCB2 could improve oocyte viability and further development after parthenogenetic activation. A subsequent study indicated that PCB2 effectively attenuated vitrification-induced oxidative stress, rescued mitochondrial dysfunction, and improved cell viability. Moreover, PCB2 also acts as a cortical tension regulator apart from strong antioxidant properties. Increased cortical tension caused by PCB2 would maintain normal spindle morphology and promote migration, ensure correct meiosis progression and finally reduce the aneuploidy rate in vitrified oocytes. Further study reveals that ATP biosynthesis plays a crucial role in cortical tension regulation, and PCB2 effectively increased the cortical tension through the electron transfer chain pathway. Additionally, PCB2 would elevate the cortical tension in embryo cells at morula and blastocyst stages and further improve blastocyst quality. What's more, targeted metabolomics shows that PCB2 has a beneficial effect on blastocyst formation by mediating saccharides and amino acids metabolism. Conclusions Antioxidant PCB2 exhibits multi-protective roles in response to vitrification stimuli through mitochondria-mediated cortical tension regulation.
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