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Combined treatment with IGF1, CoQ10, and melatonin improves the quality of bovine oocytes and heat-shocked blastocysts†

文献类型： 外文期刊

作者： Zhang, Hang 1 ; Yang, Baigao 1 ; Zhang, Peipei 2 ; Cao, Jianhua 1 ; Zhang, Xiaomeng 1 ; Gahallah, Smhaa 3 ; Roth, Zvi 5 ; Wan, Pengcheng 6 ; Zhao, Xueming 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Anim Sci IAS, 2 Yuanmingyuan Western Rd, Beijing 100193, Peoples R China

2.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin, Peoples R China

3.Univ Coll Dublin, Sch Med & Med Sci, Hlth Sci Ctr, Dublin, Ireland

4.Aswan Univ, Fac Vet Med, Aswan, Egypt

5.Hebrew Univ Jerusalem, Fac Agr Food & Environm, Dept Anim Sci, Rehovot, Israel

6.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi, Xinjiang, Peoples R China

关键词： IGF1; CoQ10; melatonin; bovine; oocyte; embryo; heat shock

期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：3.0； 五年影响因子：3.5 ）

ISSN： 0006-3363

年卷期： 2025 年 113 卷 2 期

页码：

收录情况： SCI

摘要： Heat stress significantly impairs cattle fertility, particularly affecting oocytes and developing blastocysts. Developing strategies to improve embryonic development under heat stress is crucial for advancing the cattle breeding industry. This study evaluated the effects of a combined treatment with insulin-like growth factor 1 (IGF1), coenzyme Q10 (CoQ10), and melatonin (MT) (i.e., IGF1 + CoQ10 + MT) on oocyte competence and gene expression in the formed blastocysts. Bovine in vitro fertilization blastocysts were assessed under heat shock (41 degrees C for 12 h) and control conditions (38.5 degrees C). The combined supplementation of IGF1 (100 ng/mL), CoQ10 (50 mu M), and MT (1 mu M) during in vitro maturation and in vitro culture improved oocyte quality and competence by reducing reactive oxygen species levels and apoptosis, increasing mitochondrial membrane potential (Delta Psi m), optimizing mitochondrial distribution, and upregulating quality-related genes. Heat shock decreased adenosine triphosphate (ATP) content, increased apoptosis, and affected gene expression related to development, apoptosis, ubiquitination, and autophagy, impacting blastocyst development. The combined treatment mitigated heat shock damage by enhancing ATP content, reducing apoptosis, and improving gene expression, thus improving blastocyst quality. Sentence: The combined treatment with IGF1, CoQ10, and MT improves bovine oocyte and embryo developmental competence and mitigates heat shock-induced damage in IVF blastocysts.

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