文献类型： 外文期刊
作者： Wang, Yifan 1 ; Xu, Liwen 3 ; Ma, Weixiang 1 ; Sun, Heng 1 ; Huang, Zengchao 1 ; Cai, Shuanghu 1 ; Jian, Jichang 1 ; Huan 1 ;
作者机构： 1.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Fisheries Coll, Zhanjiang, Peoples R China
2.Guangdong Ocean Univ, Key Lab Control Dis Aquat Econ Anim Guangdong Hig, Fisheries Coll, Zhanjiang, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China
关键词： betanodavirus; Murray cod; reverse transcriptase polymerase chain reaction; viral nervous necrosis
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期：
页码：
收录情况： SCI
摘要： In December 2019, a mass mortality among cultured Murray cod (Maccullochella peelii peelii) fry occurred on a freshwater farm located at Foshan city of Guangdong province, China. The cumulative mortality was up to 45% within 15 days. The diseased fish showed clinical signs, including abnormal swimming behaviour, loss of appetite and dark body colouration before mass mortality. Samples of brain and retina tissues were collected from affected fish and subjected to reverse transcriptase polymerase chain reaction detection and virus isolation in cell culture. Approximately 430 bp product was detected from the brain and retina tissues and culture supernatant of betanodavirus-infected SSN-1 cells. The typical cytopathic effect of betanodavirus infection, which is characterized by vacuolation, was observed in SSN-1 cells at three days after inoculating with the tissue filtrate of diseased Murry cod fry, and the TCID50 of the infected SSN-1 cell supernatant was 10(7.8). Histopathological examinations revealed vacuolation and necrosis in the brain and retina of naturally and experimentally infected Murray cod fry. Electron microscopic observation also showed the aggregation of numerous spherical, non-enveloped viral particles measuring 22-28 nm in diameter in the cytoplasm of betanodavirus-infected SSN-1 cells. Sequence and phylogenetic analysis based on RdRp and Cp genes further indicated that the betanodavirus isolated from Murray cod belonged to the RGNNV genotype. Much higher mortality was obtained in challenged Murray cod fry compared with the controls through immersion challenge. This study is the first report of the natural infection of betanodavirus in freshwater fish in China.
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