您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

First insight into how stress exposure triggers Vibrio harveyi recipient successful conjugation

文献类型： 外文期刊

作者： Deng, Yiqin 1 ; Gao, Si'ao 1 ; Xu, Liwen 1 ; Cheng, Changhong 1 ; Ma, Hongling 1 ; Feng, Juan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China

关键词： Vibrio harveyi; stress exposure; horizontal gene transfer; induced fertility; environmental regulation

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：3.7； 五年影响因子：4.7 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： Conjugation is the most common horizontal gene transfer (HGT) process that can be affected by environmental change and promote bacterial virulence and drug resistance. However, it is unknown whether environmental changes can influence the conjugation ability of the marine fish pathogen Vibrio harveyi, thereby affecting its pathogenicity and drug resistance. This study systematically analyzes the effect of environmental stress on the ability of V. harveyi to obtain shuttle plasmids from Escherichia coli during conjugation. The results indicate that V. harveyi cannot receive shuttle plasmid pMMB207 without exposure to stress. However, certain stress exposure (37-46 degrees C, 4%-16% ethanol, 0.14-0.56 mM SDS, 0.04 similar to 0.05 M NaOH, and 0.012-0.024 M HCl for 5-60 minutes) in the log phase of V. harveyi before conjugation successfully induces the fertility of the V. harveyi recipient in intergeneric mating with E. coli. In particular, ethanol and heat stress showed strong induction with up to 2.5 x 10(5) and 5.3 x 10(3) transconjugants when exposed to 16% ethanol for 10 minutes and 40 degrees C for 60 minutes, respectively. Additionally, appropriate levels of NaOH (0.05 M, 10 minutes), SDS (0.42 mM, 5 minutes), and HCl (0.024 M, 5 minutes) lead to 2.3 x 10(3), 4.5x10(2), and 1.8 x 10(2) transconjugants, respectively. These results will help establish homologous recombination gene knockout technology and greatly advance molecular theoretical research on V. harveyi. They will also support the establishment of disease prevention and control strategies based on the interruption of the HGT process by environmental regulation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台