A ferritin nanoparticle vaccine based on the hemagglutinin extracellular domain of swine influenza A (H1N1) virus elicits protective immune responses in mice and pigs
文献类型： 外文期刊
作者： Tang, Pan 1 ; Cui, Enhui 2 ; Cheng, Jinghua 1 ; Li, Benqiang 1 ; Tao, Jie 1 ; Shi, Ying 1 ; Jiao, Jiajie 1 ; Du, Enqi 3 ; Wang, Jingyu 3 ; Liu, Huili 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai, Peoples R China
2.Shanghai Jiao Tong Univ, Instrumental Anal Ctr, Shanghai, Peoples R China
3.Northwest A&F Univ, Coll Vet Med, Yangling, Peoples R China
关键词： nanoparticle vaccine; hemagglutinin extracellular domain; protective immunity; ferritin; swine influenza virus
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.3； 五年影响因子：8.0 ）
ISSN： 1664-3224
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Introduction Swine influenza viruses (SIVs) pose significant economic losses to the pig industry and are a burden on global public health systems. The increasing complexity of the distribution and evolution of different serotypes of influenza strains in swine herds escalates the potential for the emergence of novel pandemic viruses, so it is essential to develop new vaccines based on swine influenza.Methods Here, we constructed a self-assembling ferritin nanoparticle vaccine based on the hemagglutinin (HA) extracellular domain of swine influenza A (H1N1) virus using insect baculovirus expression vector system (IBEVS), and after two immunizations, the immunogenicities and protective efficacies of the HA-Ferritin nanoparticle vaccine against the swine influenza virus H1N1 strain in mice and piglets were evaluated.Results Our results demonstrated that HA-Ferritin nanoparticle vaccine induced more efficient immunity than traditional swine influenza vaccines. Vaccination with the HA-Ferritin nanoparticle vaccine elicited robust hemagglutinin inhibition titers and antigen-specific IgG antibodies and increased cytokine levels in serum. MF59 adjuvant can significantly promote the humoral immunity of HA-Ferritin nanoparticle vaccine. Furthermore, challenge tests showed that HA-Ferritin nanoparticle vaccine conferred full protection against lethal challenge with H1N1 virus and significantly decreased the severity of virus-associated lung lesions after challenge in both BALB/c mice and piglets.Conclusion Taken together, these results indicate that the hemagglutinin extracellular-based ferritin nanoparticle vaccine may be a promising vaccine candidate against SIVs infection.
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