The lignin-degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR/Cas9
文献类型： 外文期刊
作者： Nakazawa, Takehito 1 ; Inoue, Chikako 1 ; Morimoto, Ryota 1 ; Nguyen, Dong Xuan 1 ; Bao, Dapeng 2 ; Kawauchi, Moriyuki 1 ; Sakamoto, Masahiro 1 ; Honda, Yoichi 1 ;
作者机构： 1.Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan
2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China
3.Kyoto Univ, Grad Sch Agr, Oiwake Cho,Sakyo Ku, Kyoto 6068502, Japan
期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.1； 五年影响因子：5.8 ）
ISSN： 1462-2912
年卷期： 2023 年 25 卷 8 期
页码：
收录情况： SCI
摘要： White-rot fungi efficiently degrade wood lignin; however, the mechanisms involved remain largely unknown. Recently, a forward genetics approach to identify several genes in Pleurotus ostreatus (Agaricales) in which mutations cause defects in wood lignin degradation was used. For example, pex1 encodes a peroxisome biogenesis factor and gat1 encodes a putative Agaricomycetes-specific DNA-binding transcription factor. In this study, we examined the effects of single-gene mutations in pex1 or gat1 on wood lignin degradation in another white-rot fungus, Gelatoporia (Ceriporiopsis) subvermispora (Polyporales), to investigate conserved and derived degradation mechanisms in white-rot fungi. G. subvermispora pex1 and gat1 single-gene mutant strains were generated from a monokaryotic wild-type strain, FP-90031-Sp/1, using plasmid-based CRISPR/Cas9. As in P. ostreatus, Gsgat1 mutants were nearly unable to degrade lignin sourced from beech wood sawdust medium (BWS), while Gspex1 mutants exhibited a delay in lignin degradation. We also found that the transcripts of lignin modifying enzyme-encoding genes, mnp4, mnp5, mnp6, mnp7, and mnp11, which predominantly accumulate in FP-90031-Sp/1 cultured with BWS, were greatly downregulated in Gsgat1 mutants. Taken together, the results suggest that Gat1 may be a conserved regulator of the ligninolytic system of white-rot fungi and that the contribution of peroxisomes to the ligninolytic system may differ among species.
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