Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus
文献类型： 外文期刊
作者： Yu, Shuhui 1 ; Wang, Ying 2 ; Wu, Yingying 2 ; Bao, Dapeng 2 ; Bing, Wei 1 ; Li, Yan 2 ; Chen, Hongyu 2 ;
作者机构： 1.Changchun Univ Technol, Sch Chem & Life Sci, Changchun 130012, Peoples R China
2.Shanghai Acad Agr Sci, Minist Sci & Technol MOST, Minist Agr, Natl Engn Res Ctr Edible Fungi,Inst Edible Fungi,K, Shanghai 201403, Peoples R China
3.Jilin Univ, Key Lab Bion Engn, Minist Educ, Changchun 130022, Peoples R China
关键词： Hypsizygus marmoreus; fungal immunomodulatory protein; protein structure; phylogenetic tree; recombinant expression; cytokine release
期刊名称：MOLECULES （ 影响因子：4.6； 五年影响因子：4.9 ）
ISSN：
年卷期： 2023 年 28 卷 12 期
页码：
收录情况： SCI
摘要： A novel fungal immunomodulatory protein (FIP), identified as FIP-hma, was discovered in the genome of an edible mushroom Hypsizygus marmoreus. Bioinformatics analysis suggested FIP-hma contained the cerato-platanin (CP) conserved domain and was categorized into Cerato-type FIP. In phylogenetic analysis, FIP-hma was clustered into a new branch of the FIP family, displaying large system divergence from most of the other FIPs. The higher gene expression of FIP-hma was observed during the vegetative growth stages than that during the reproductive growth stages. In addition, the cDNA sequence of FIP-hma was cloned and successfully expressed in Escherichia coli (E. coli) BL21(DE3). The recombinant protein of FIP-hma (rFIP-hma) was neatly purified and isolated by Ni-NTA and SUMO-Protease. The iNOS, IL-6, IL-1 & beta;, and TNF-& alpha; levels of RAW 264.7 macrophages were upregulated by rFIP-hma, indicating its activation of an immune response by regulating central cytokines. No cytotoxic effects were observed in an MTT test. The findings of this work discovered a novel immunoregulatory protein from H. marmoreus, provided a systematic bioinformatic profile, suggested an effective approach for its heterologous recombinant production, and reported its potent immunoregulatory activity in macrophages. This study sheds light on the physiological function research of FIPs and their further industrial utilization.
- 相关文献
作者其他论文 更多>>
-
Synergizing postharvest physiology and nanopackaging for edible mushroom preservation
作者：Gong, Ming;Zhang, Tongyan;Wu, Yingying;Shang, Junjun;Gong, Ming;Su, Erzheng;Cao, Yu;Gong, Ming;Zhang, Tongyan;Zhang, Jianguo
关键词：Edible fungi; Postharvest preservation; Physiological changes; Nanopackaging; Food nanotechnology
-
Effectiveness of Different Organic Solvent Additions to Water Samples for Reducing the Adsorption Effects of Organic Pesticides Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Liu, Yucan;Xu, Xinyi;Wang, Ying;Zhang, Yan;Lu, Jianbo;Liu, Chengbin;Duan, Jinming;Sun, Hongwei
关键词：adsorption effect; UHPLC-ESI-MS/MS; organic pesticides; direct injection technique; detection signal intensity; organic solvent; addition ratio
-
OsMAPKKK5 affects brassinosteroid signal transduction via phosphorylating OsBSK1-1 and regulates rice plant architecture and yield
作者：Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Wang, Ying;Wang, Ying;Hu, Zejun;Niu, Fuan;Cao, Liming;Liu, Yang;Luo, Xiaojin
关键词：brassinosteroids; mitogen-activated protein kinase kinase kinase; crosstalk; plant architecture; rice yield
-
Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase
作者：Wang, Ying;Chen, Wenjing;Chen, Hongyu;Gong, Ming;Shao, Youran;Bao, Dapeng;Zou, Gen;Chen, Wenjing;Wang, Li
关键词：Mycoprotein; CRISPR/Cas9; Molecular design breeding; Endo; N -acetyl-beta-D-glucosaminidase; Cordyceps militaris
-
Characterization and Functional Analysis of a Novel Fungal Immunomodulatory Protein Gene from Ganoderma leucocontextum in B16-F10 Mouse Melanoma Cells
作者：Yang, Jiayi;Jin, Mengyuan;Zhang, Lida;Zhou, Xuanwei;Wu, Yingying
关键词：
Ganoderma leucocontextum ; fungal immunomodulatory proteins; protein expression; B16-F10 melanoma cell -
The Exploration of Cordyceps militaris Extract as a Postharvest Preservative for Flammulina filiformis
作者：Chen, Wenjing;Chen, Wenjing;Li, Yan;Shao, Youran;Chen, Hongyu;Gong, Ming;Wang, Ying;Zou, Gen;Wang, Li;Li, Nanyi
关键词：
Flammulina filiformis ;Cordyceps militaris ; bacteriostasis; antioxidation; preservation -
Multilayered regulation of autophagy-related protein kinase in Cordyceps militaris
作者：Wang, Yating;Li, Nanyi;Wang, Ying;Li, Bing;Shao, Youran;Chen, Hongyu;Gong, Ming;Zhang, Ruijing;Liu, Yu;Chen, Wenjing;Zou, Gen
关键词：Cordyceps militaris; Autophagy; Fruiting body; Pathogenicity; Secondary metabolism
