Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase
文献类型： 外文期刊
作者： Wang, Ying 1 ; Chen, Wenjing 1 ; Chen, Hongyu 1 ; Gong, Ming 1 ; Shao, Youran 1 ; Wang, Li 3 ; Bao, Dapeng 1 ; Zou, Gen 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, 999 Huchenghuan Rd, Shanghai 201306, Peoples R China
3.Pureway Biotechnol Ltd, 1065 West Zhongshan Rd, Shanghai 200051, Peoples R China
关键词： Mycoprotein; CRISPR/Cas9; Molecular design breeding; Endo; N -acetyl-beta-D-glucosaminidase; Cordyceps militaris
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 305 卷
页码：
收录情况： SCI
摘要： Fungi comprise approximately 2 % of the Earth's biomass; however, the human gastrointestinal tract has a limited capacity to digest fungal biomass. In this study, a novel endo-N-acetyl-beta-D-glucosaminidase, Endo CM, was characterized in the mushroom-forming fungus Cordyceps militaris, where it plays a role in maintaining the integrity of the fungal cell wall. Through gene editing, the Endo CM promoter was engineered to remove the binding site of the CmCreA carbon catabolite repressor, and the transformant was named CmT. After 12 h of treatment with simulated digestive fluids, the residual mycelial biomass of CmT was reduced to 50.00 +/- 1.57 %, compared with 69.47 +/- 0.97 % (p = 0.00005) for the parent strain. CmT also released more amino acids during the simulated digestion, suggesting that the expression level of Endo CM affects the accessibility of mycelial biomass to digestive enzymes. Additionally, CmT produced fruiting bodies with improved flavor but impaired appearance. This study highlights the production of alternative proteins with high digestibility and provides a sustainable approach for breeding mushrooms with improved digestibility and absorption properties.
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