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De novo gonad transcriptome analysis of elongate loath (Leptobotia elongata) provides novel insights into sex-related genes

文献类型： 外文期刊

作者： Zhang, Yunbang 1 ; Cao, Xiaojuan 1 ; Zou, Yuanchao 3 ; Yan, Ze 1 ; Huang, Yuwei 1 ; Zhu, Yurong 1 ; Gao, Jian 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Engn Res Ctr Green Dev Convent Aquat Biol Ind Yan, Minist Educ, Wuhan 430070, Peoples R China

2.Hubei Prov Engn Lab Pond Aquaculture, Wuhan, Hubei, Peoples R China

3.Neijiang Normal Univ, Coll Life Sci, Conservat & Utilizat Fishes Resources Upper Reach, Neijiang 641100, Sichuan, Peoples R China

4.CAFS, Yangtze River Fishery Res Inst, Minist Agr, Fishery Resources & Environm Sci Expt Stn Upper M, Wuhan 430223, Peoples R China

关键词： Endangered species; Leptobotia elongata; Sex-related genes; Transcriptome; Testis and ovary

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：3.306； 五年影响因子：3.251 ）

ISSN： 1744-117X

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： Elongate loath (Leptobotia elongata) is endemic in middle and upper reaches of the Yangtze River in China. Because of many anthropogenic factors such as overfishing and dam construction, the loath has become a highly endangered species. So far, the genomic resources which benefit for species conservation and utilization are still lacking in elongate loath. Therefore, the first gonad transcriptome analysis of the loath was conducted in this study, providing novel insights into sex-related genes. A total of 286,800,660 clean reads with a total length of 42.02 Gb were obtained. 18,975 differentially expressed genes (DEGs) were identified, where 12,976 DEGs, especially Sox9a, Sox9b, Igf2 and Fgfr2, were upregulated in the testis, and 5999 DEGs, especially Zp3, Eg2, Plk1, Ccnb1, Cdc20 and Mos, were upregulated in the ovary. Meanwhile, some testis-specific genes (i.e. Cald1, Atp1a, Muc2 and Cat) and ovary-specific genes (i.e. Ca4, Tuba, Acp5, Ccna, Larp6 and Nop4) were identified and verified. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of DEGs, we found a series of enrichment pathways related to reproduction in elongate loath, such as the MAPK signaling pathway, oxytocin signaling pathway and oocyte meiosis pathway. Twelve DEGs were randomly selected to verify RNA-seq results by qPCR. In conclusion, this study provides a data source to study the molecular characteristics and regulatory mechanisms of sex-related genes in elongate loath, which has a potential to improve the resource protection and aquaculture production of the loath.

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