A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement
文献类型： 外文期刊
作者： Zhang, Xu 1 ; Wang, Haiyan 1 ; Sun, Haojie 1 ; Li, Yingbo 2 ; Feng, Yilong 1 ; Jiao, Chengzhi 1 ; Li, Mengli 1 ; Song, Xinying 1 ; Wang, Tong 1 ; Wang, Zongkuan 1 ; Yuan, Chunxia 1 ; Sun, Li 1 ; Lu, Ruiju 2 ; Zhang, Wenli 1 ; Xiao, Jin 1 ; Wang, Xiue 1 ;
作者机构： 1.Nanjing Agr Univ, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, JCIC MCP, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Biotech Res Inst, Shanghai 201106, Peoples R China
关键词： D; villosum genome; crop wild relative; genome evolution; disease resistance; RNA-seq; MNase hy-persensitivity assay
期刊名称：MOLECULAR PLANT （ 影响因子：27.5； 五年影响因子：22.6 ）
ISSN： 1674-2052
年卷期： 2023 年 16 卷 2 期
页码：
收录情况： SCI
摘要： Dasypyrum villosum is one of the most valuable gene resources in wheat improvement, especially for dis-ease resistance. The mining of favorable genes from D. villosum is frustrated by the lack of a whole genome sequence. In this study, we generated a doubled-haploid line, 91C43DH, using microspore culture and ob-tained a 4.05-GB high-quality, chromosome-scale genome assembly for D. villosum. The assembly con-tains39 727 high-confidence genes, and 85.31% of the sequences are repetitive. Two reciprocal transloca-tion events were detected, and 7VS-4VL is a unique translocation in D. villosum. The prolamin seed storage protein-coding genes were found to be duplicated; in particular, the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded. RNA sequencing (RNA-seq) analysis indicated that, afterBlumeria graminearum f.sp tritici (Bgt) inoculation, there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D. villosum than in Triticum urartu. MNase hypersensitive sequencing (MH-seq) identified two Bgt-inducible MH sites (MHSs), one in the promoter and one in the 30 terminal region of the powdery mildew resistance (Pm) gene NLR1-V. Each site had two subpeaks and they were termed MHS1 (MHS1.1/1.2) and MHS2 (MHS2.1/2.2). Bgt- inducible MHS2.2 was uniquely present in D. villosum, and MHS1.1 was more inducible in D. villosum than in wheat, suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense. In summary, this study provides a valuable genome resource for functional genomics studies and wheat-D. villosum introgression breeding. The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.
- 相关文献
作者其他论文 更多>>
-
Machine learning prediction of biochar physicochemical properties based on biomass characteristics and pyrolysis conditions
作者：Song, Yuanbo;Huang, Zipeng;Jin, Mengyu;Liu, Zhe;Wang, Xiaoxia;Shen, Zheng;Zhang, Yalei;Hou, Cheng;Zhang, Xu;Zhang, Yalei;Zhang, Xu;Shen, Zheng;Zhang, Yalei
关键词：Biochar; Pyrolysis; EXtreme gradient boosting model; Elemental distribution; Aromaticity
-
Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.)
作者：Gao, Runhong;Zong, Yingjie;Zhang, Shuwei;Guo, Guimei;Zhang, Wenqi;Chen, Zhiwei;Lu, Ruiju;Liu, Chenghong;Wang, Yifei;Li, Yingbo
关键词：Isolated microspore culture; Oryza sativa L.; Low-temperature pre-treatment; Microspore developmental stage; Callus induction; Plantlet regeneration
-
Nitrogen removal performance and mechanism in constructed wetlands under saline conditions: Role of Canna indica inoculated with Piriformospora indica
作者：Cui, Naxin;Cai, Min;Zhang, Xu;Zeng, Rong;Zhou, Li;Chen, Guifa;Zou, Guoyan;Cui, Naxin;Cai, Min;Zhang, Xu;Zeng, Rong;Zhou, Li;Chen, Guifa;Zou, Guoyan;Cui, Naxin;Zeng, Rong
关键词：Phytoremediation; Salt stress; Rhizosphere microbe; Plant uptake; Growth-promoting fungus
-
The aging of microplastics exacerbates the damage to photosynthetic performance and bioenergy production in microalgae (Chlorella pyrenoidosa)
作者：Xu, Yazhou;Zhang, Yalei;Xu, Yazhou;Peng, Bo-Yu;Zhang, Xu;Xu, Qianfeng;Yang, Libin;Chen, Jiabin;Zhou, Xuefei;Zhang, Yalei;Zhang, Xu;Chen, Jiabin;Zhou, Xuefei;Zhang, Yalei;Zhang, Yalei
关键词：Microplastics; Aging; Polyvinyl chloride; Microalgae; Photosynthetic performance; Oxidative stress
-
Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores
作者：Li, Yingbo;Zong, Yingjie;Li, Wenrui;Guo, Guimei;Zhou, Longhua;Xu, Hongwei;Gao, Runhong;Liu, Chenghong;Li, Yingbo;Zong, Yingjie;Li, Wenrui;Guo, Guimei;Zhou, Longhua;Xu, Hongwei;Gao, Runhong;Liu, Chenghong
关键词：Microspore embryogenesis; Cold stress; Rice; Transcriptome; Metabolomics
-
Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (Hordeum vulgare L.) Leaves Derived from Mutagenesis Combined with Microspore Culture
作者：Xu, Hongwei;Xu, Rugen;Xu, Hongwei;Guo, Guimei;Chen, Zhiwei;Li, Yingbo;Zhou, Longhua;Liu, Chenghong;Halford, Nigel G.
关键词：barley; mutagenesis; salt tolerance; microspore; transcriptome; metabolome
-
Isoprenylation modification is required for HIPP1-mediated powdery mildew resistance in wheat
作者：Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Chen, Yiming;Tang, Xiong;Zhao, Jia;Yu, Feifei;Wang, Haiyan;Xiao, Jin;Liu, Jia;Zhang, Xu;Sun, Li;Xie, Qi;Wang, Xiue;Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Zhang, Heng;Li, Yingbo
关键词：heavy-metal-associated protein; plant defence signalling; protein isoprenylation modification; Triticum aestivum L
