A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement
文献类型： 外文期刊
作者： Zhang, Xu 1 ; Wang, Haiyan 1 ; Sun, Haojie 1 ; Li, Yingbo 2 ; Feng, Yilong 1 ; Jiao, Chengzhi 1 ; Li, Mengli 1 ; Song, Xinying 1 ; Wang, Tong 1 ; Wang, Zongkuan 1 ; Yuan, Chunxia 1 ; Sun, Li 1 ; Lu, Ruiju 2 ; Zhang, Wenli 1 ; Xiao, Jin 1 ; Wang, Xiue 1 ;
作者机构： 1.Nanjing Agr Univ, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, JCIC MCP, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Biotech Res Inst, Shanghai 201106, Peoples R China
关键词： D; villosum genome; crop wild relative; genome evolution; disease resistance; RNA-seq; MNase hy-persensitivity assay
期刊名称：MOLECULAR PLANT （ 影响因子：27.5； 五年影响因子：22.6 ）
ISSN： 1674-2052
年卷期： 2023 年 16 卷 2 期
页码：
收录情况： SCI
摘要： Dasypyrum villosum is one of the most valuable gene resources in wheat improvement, especially for dis-ease resistance. The mining of favorable genes from D. villosum is frustrated by the lack of a whole genome sequence. In this study, we generated a doubled-haploid line, 91C43DH, using microspore culture and ob-tained a 4.05-GB high-quality, chromosome-scale genome assembly for D. villosum. The assembly con-tains39 727 high-confidence genes, and 85.31% of the sequences are repetitive. Two reciprocal transloca-tion events were detected, and 7VS-4VL is a unique translocation in D. villosum. The prolamin seed storage protein-coding genes were found to be duplicated; in particular, the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded. RNA sequencing (RNA-seq) analysis indicated that, afterBlumeria graminearum f.sp tritici (Bgt) inoculation, there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D. villosum than in Triticum urartu. MNase hypersensitive sequencing (MH-seq) identified two Bgt-inducible MH sites (MHSs), one in the promoter and one in the 30 terminal region of the powdery mildew resistance (Pm) gene NLR1-V. Each site had two subpeaks and they were termed MHS1 (MHS1.1/1.2) and MHS2 (MHS2.1/2.2). Bgt- inducible MHS2.2 was uniquely present in D. villosum, and MHS1.1 was more inducible in D. villosum than in wheat, suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense. In summary, this study provides a valuable genome resource for functional genomics studies and wheat-D. villosum introgression breeding. The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.
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