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Screening and analysis of candidate genes conferring alkalinity tolerance in rice (Oryza sativa L.) at the bud burst stage based on QTL-seq and RNA-seq

文献类型： 外文期刊

作者： Wang, Jiangxu 1 ; Bian, Jingyang 4 ; Liu, Linshuai 4 ; Gao, Shiwei 5 ; Liu, Qing 5 ; Feng, Yanjiang 6 ; Shan, Lili 6 ; Guo, Junxiang 6 ; Wang, Guiling 6 ; Sun, Shichen 1 ; Jiang, Hui 1 ; Chen, Lei 1 ; Lei, Lei 1 ; Liu, Kai 1 ;

作者机构： 1.Heilongjiang Prov Acad Agr Sci, Northeast Ctr Natl Salt, Harbin 150086, Peoples R China

2.Heilongjiang Prov Acad Agr Sci, Alkali Tolerant Rice Technol Innovat Ctr, Harbin 150086, Peoples R China

3.Heilongjiang Acad Agr Sci, Postdoctoral Workstn, Harbin 150086, Peoples R China

4.Heilongjiang Acad Agr Sci, Daqing Branches, Daqing 163000, Peoples R China

5.Heilongjiang Acad Agr Sci, Suihua Branch, Suihua 152000, Peoples R China

6.Heilongjiang Acad Agr Sci, Rice Res Inst, Jiamusi 154000, Peoples R China

关键词： Alkalinity tolerance; Bud burst; Candidate genes; Cropland; Oryza sativa L; QTL-seq; Rice; RNA-seq; Soil salinization

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.3； 五年影响因子：2.5 ）

ISSN： 0717-3458

年卷期： 2024 年 71 卷

页码：

收录情况： SCI

摘要： Background: Soil salinization is one of the key factors restricting the production of cropland. Once rice is subjected to alkali stress at the bud burst stage, the yield will suffer irreparable serious loss. Compared with salt tolerance, studies on QTL mapping and candidate gene analysis of rice alkali tolerance are limited. Results: In this study, we used the F 2:3 population derived from the alkali-tolerant cultivar LD21 and the alkali-sensitive cultivar WL138 to construct an alkali-tolerant DNA mixing pool, and the BSA (Bulked Segregation Analysis) method was used for re-sequencing. The main QTL qRSLB9 controlling the relative shoot length of rice under alkali stress was mapped by QTL-seq. The candidate interval was narrowed to 346.5 kb by regional linkage mapping, which containing 6 DEGs screened through transcriptome sequencing. The qRT-PCR and candidate gene sequencing showed that LOC_Os09g24260 was most likely to control relative shoot length (RSL) in rice as a major gene who encodes the WD domain, G-beta repeat domain-containing protein. Conclusions: Based on these results, LOC_Os09g24260 was the candidate gene of qRSLB9 conferring alkalinity tolerance to rice at the bud burst stage. Our study provides valuable genetic information and materials for breeding new rice varieties with alkalinity tolerance. (c) 2024 The Authors. Published by Elsevier Inc. on behalf of Pontificia Universidad Cat & oacute;lica de Valparaiso. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-ncnd/4.0/).

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