文献类型： 外文期刊
作者： Zhang, Cuimei 1 ; Tang, Yajun 2 ; Tang, Shanjie 3 ; Chen, Lei 3 ; Li, Tong 3 ; Yuan, Haidi 2 ; Xu, Yujun 1 ; Zhou, Yangyan 1 ; Zhang, Shuaibin 3 ; Wang, Jianli 4 ; Wen, Hongyu 5 ; Jiang, Wenbo 5 ; Pang, Yongzhen 5 ; Deng, Xian 3 ; Cao, Xiaofeng 3 ; Zhou, Junhui 2 ; Song, Xianwei 3 ; Liu, Qikun 1 ;
作者机构： 1.Peking Univ, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
2.Peking Univ Inst Adv Agr Sci, Shandong Lab Adv Agr Sci Weifang, Weifang 261000, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China
4.Heilongjiang Acad Agr Sci, Grass & Sci Inst, Harbin 150086, Peoples R China
5.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
6.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Seed Innovat, Beijing 100101, Peoples R China
关键词： inducible CRISPR activation; morphogenic gene; genetic transformation; plant regeneration
期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）
ISSN： 2590-3462
年卷期： 2024 年 5 卷 5 期
页码：
收录情况： SCI
摘要： The inducible CRISPR activation (CRISPR-a) system offers unparalleled precision and versatility for regulating endogenous genes, making it highly sought after in plant research. In this study, we developed a chemically inducible CRISPR-a tool for plants called ER-Tag by combining the LexA-VP16-ER inducible system with the SunTag CRISPR-a system. We systematically compared different induction strategies and achieved high efficiency in target gene activation. We demonstrated that guide RNAs can be multiplexed and pooled for large-scale screening of effective morphogenic genes and gene pairs involved in plant regeneration. Further experiments showed that induced activation of these morphogenic genes can accelerate regeneration and improve regeneration efficiency in both eudicot and monocot plants, including alfalfa, woodland strawberry, and sheepgrass. Our study expands the CRISPR toolset in plants and provides a powerful new strategy for studying gene function when constitutive expression is not feasible or ideal.
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