Mechanistic elucidation of cyanamide's selective herbicidal activity via integrated transcriptome-metabolome analysis: Implications for cleaner alfalfa production
文献类型： 外文期刊
作者： Sun, Weihong 1 ; Wang, Xianguo 1 ; Wang, Jianli 3 ; Guo, Junhong 4 ; An, Mingzhu 1 ; Geng, Feilong 1 ; Ren, Wei 1 ;
作者机构： 1.China Agr Univ, Coll Grassland Sci & Technol, Beijing 100193, Peoples R China
2.Weifang Univ Sci & Technol, Jia Sixie Agr Coll, Weifang 262700, Peoples R China
3.Heilongjiang Acad Agr Sci, Pratacultural Sci Inst, Harbin 161499, Peoples R China
4.Shandong Second Med Univ, Sch Anesthesiol, Weifang 261053, Peoples R China
关键词： Cyanamide stress resistance; Alfalfa; Redroot pigweed; Transcription-associated metabolomic analysis
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2025 年 229 卷
页码：
收录情况： SCI
摘要： Redroot pigweed (Amaranthus retroflexus L.) is one of the common weeds in alfalfa (Medicago sativa L.) field, compromising yield and forage quality. Cyanamide (CA), a phytogenic herbicide, effectively suppresses redroot pigweed without harming alfalfa, yet its molecular mechanisms remain elusive. This study employed an integrated transcriptomic and metabolomic approach to analyze leaves of redroot pigweed and alfalfa treated with and without CA spraying. This multi-angled investigation elucidates the mechanisms underlying CA's effects on the growth of both plant species. Integrated transcriptomic and metabolomic analyses revealed that CA upregulates genes linked to indole-3-acetic acid (IAA) biosynthesis (MPK9, MPK3, MPK6, WRKY33) and key enzymes (tryptophan aminotransferase, acetaldehyde amine dehydratase, indoleacetic acid dehydrogenase) in alfalfa, enhancing stress tolerance. Conversely, CA downregulates genes (Cluster-14830.0, Cluster-17212.0, Cluster-21179.2, Cluster-26728.0) and enzymes (branched-chain amino acid aminotransferase, acetolactate synthase, dihydroxy-acid dehydratase) in redroot pigweed, disrupting valine, leucine, and isoleucine biosynthesis and inducing leaf wilting. These findings elucidate CA's dual role in alfalfa defense and weed suppression, supporting its use as a sustainable bioherbicide.
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