Identification of the Cold-Related Genes COLD11 and OsCTS11 via BSA-seq and Fine Mapping at the Rice Seedling Stage
文献类型： 外文期刊
作者： Wu, Bian 1 ; Fu, Minghui 1 ; Du, Jinghua 1 ; Wang, Mengjing 1 ; Zhang, Siyue 1 ; Li, Sanhe 1 ; Chen, Junxiao 1 ; Zha, Wenjun 1 ; Li, Changyan 1 ; Liu, Kai 1 ; Xu, Huashan 1 ; Wang, Huiying 1 ; Shi, Shaojie 1 ; Wu, Yan 1 ; Li, Peide 1 ; You, Aiqing 1 ; Zhou, Lei 1 ;
作者机构： 1.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan 430070, Peoples R China
2.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
3.Huazhong Agr Univ, Wuhan 430070, Peoples R China
关键词：
Rice; Cold Stress; BSA-seq;
期刊名称：RICE （ 影响因子：5.0； 五年影响因子：5.5 ）
ISSN： 1939-8425
年卷期： 2024 年 17 卷 1 期
页码：
收录情况： SCI
摘要： Cold stress has a significantly negative effect on the growth, development, and yield of rice. However, the genetic basis for the differences in the cold tolerance of Xian/indica and Geng/japonica rice seedlings is still largely unknown. In this study, an RIL population was generated by crossing of the cold-tolerant japonica variety Nipponbare and the cold-sensitive indica variety WD16343 for BSA-seq analysis, and a major cold tolerance QTL qCTS11 was identified on chromosome 11. This locus was narrowed to the 584 kb region through fine mapping. Sequence alignment and expression analysis identified the cloned gene COLD11 and a novel cold-related gene OsCTS11. According to the reported functional variation of COLD11, Nipponbare (TCG + 3GCG)x2 presented more GCG repeats in the 1st exon than WD16343 (TCG + 3GCG), partially explaining the difference in cold tolerance between the parents. OsCTS11, encoding a stress enhanced protein based on phylogenetic analysis, was strongly induced by cold stress and located in the chloroplast and the nucleus. oscts11-mutant lines generated via CRISPR/Cas9 system improved the cold tolerance of rice seedlings. Our study not only reveals novel genetic loci associated with cold tolerance, but also provides potentially valuable gene resources for the cultivation of cold-tolerant rice.
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