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Metabolic engineering of artificially modified transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids

文献类型： 外文期刊

作者： Jia, Entong 1 ; Li, He 1 ; He, Fang 1 ; Xu, Xiaoyu 1 ; Wei, Jia 2 ; Shao, Gaige 3 ; Liu, Jingying 1 ; Ma, Pengda 1 ;

作者机构： 1.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China

2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Northeast Agr Res Ctr China, Changchun 130033, Peoples R China

3.Xian Agr Technol Promot Ctr, Xian 710061, Peoples R China

关键词： Salvia miltiorrhiza; Transcriptional regulation engineering; SmMYB36-VP16; Tanshinones; Phenolic acids; Elicitors; Plant cell factories

期刊名称：METABOLIC ENGINEERING （ 影响因子：6.8； 五年影响因子：7.8 ）

ISSN： 1096-7176

年卷期： 2024 年 86 卷

页码：

收录情况： SCI

摘要： Tanshinones and phenolic acids are the two main chemical constituents in Salvia miltiorrhiza, which are used clinically for the treatment of hypertension, coronary heart disease, atherosclerosis, and many other diseases, and have broad medicinal value. The efficient synthesis of the target products of these two metabolites in isolated plant tissues cannot be achieved without the regulation and optimization of metabolic pathways, and transcription factors play an important role as common regulatory elements in plant tissue metabolic engineering. However, most of the regulatory effects are specific to one class of metabolites, or an opposing regulation of two classes of metabolites exists. In this study, an artificially modified transcription factor, SmMYB36-VP16, was constructed to enhance tanshinones and phenolic acids in Salvia miltiorrhiza hair roots simultaneously. Further in combination with the elicitors dual-screening technique, by applying the optimal elicitors screened, the tanshinones content in the transgenic hairy roots of Salvia miltiorrhiza reached 6.44 mg/g DW, which was theoretically 6.08-fold that of the controls without any treatment, and the content of phenolic acids reached 141.03 mg/g DW, which was theoretically 5.05-fold that of the controls without any treatment. The combination of artificially modified transcriptional regulatory and elicitors dual-screening techniques has facilitated the ability of plant isolated tissue cell factories to produce targeted medicinal metabolites. This strategy could be applied to other species, laying the foundation for the production of potential natural products for the medicinal industry.

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