文献类型： 外文期刊

作者： Zhu, Yibin ¹ ; Cai, Haiming ¹ ; Yan, Zhuanqiang ² ; Shen, Hanqin ³ ; Fang, Siyun ² ; Wang, Dingai ² ; Liao, Shenquan ¹ ; Qi, Nanshan ¹ ; Lv, Minna ¹ ; Lin, Xuhui ¹ ; Hu, Junjing ¹ ; Song, Yongle ¹ ; Chen, Xiangjie ¹ ; Yin, Lijun ¹ ; Zhang, Jianfei ¹ ; Li, Juan ¹ ; Sun, Mingfei ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth,Minist Agr & Rural Affairs, Key Lab Livestock Dis Prevent Guangdong Prov, Key Lab Avian Influenza & Other Major Poultry Dis, Guangzhou 510640, Peoples R China

2.Wens Foodstuffs Grp Co Ltd, Wens Grp Acad, Xinxing 527400, Guangdong, Peoples R China

3.Guangdong Jingjie Inspection & Testing Co Ltd, Xinxing 527400, Guangdong, Peoples R China

关键词： Pentatrichomonas hominis; Pigs; Probiotics; Anti-oxidative response; Inflammatory response; Apoptosis

期刊名称：VETERINARY RESEARCH COMMUNICATIONS （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0165-7380

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Pentatrichomonas hominis is a common intestinal parasitic protozoan that causes abdominal pain and diarrhea, and poses a zoonotic risk. Probiotics, known for enhancing immunity and pathogen resistance, hold promise in combating parasitic infections. This study aimed to evaluate two porcine-derived probiotics, Lactobacillus reuteri LR1 and Lactobacillus plantarum LP1, against P. hominis infections in pigs. Taxonomic identity was confirmed through 16 S rRNA gene sequencing, with L. reuteri LR1 belonging to L. reuteri species and L. plantarum LP1 belonging to L. plantarum species. Both probiotics exhibited robust in vitro growth performance. Co-culturing intestinal porcine epithelial cell line (IPEC-J2) with these probiotics significantly improved cell viability compared with the control group. Pre-incubation probiotics significantly enhanced the mRNA expression of anti-oxidative response genes in IPEC-J2 cells compared with the PHGD group, with L. reuteri LR1 and L. plantarum LP1 significantly up-regulating CuZn-SOD,CAT and Mn-SOD genes expression (p < 0.05). The anti-oxidative stress effect of L. reuteri LR1 was significantly better than that of L. plantarum LP1 (p < 0.05). Furthermore, pre-incubation with the probiotics alleviated the P. hominis-induced inflammatory response. L. reuteri LR1 and L. plantarum LP1 significantly down-regulated IL-6,IL-8 and TNF-alpha gene expression(p < 0.05) compared with the PHGD group. The probiotics also mitigated P. hominis-induced apoptosis. L. reuteri LR1 and L. plantarum LP1 significantly down-regulated Caspase3 and Bax gene expression (p < 0.05), significantly up-regulated Bcl-2 gene expression (p < 0.05) compared with the PHGD group. Among them, L. plantarum LP1 showed better anti-apoptotic effect. These findings highlight the probiotics for mitigating P. hominis infections in pigs. Their ability to enhance anti-oxidative responses, alleviate inflammation, and inhibit apoptosis holds promise for therapeutic applications. Simultaneously, probiotics can actively contribute to inhibiting trichomonal infections, offering a novel approach for preventing and treating diseases such as P. hominis. Further in vivo studies are required to validate these results and explore their potential in animal and human health.