文献类型： 外文期刊

作者： Li, Lei ³ ; Han, Kaiou ³ ; Mao, Xinyu ³ ; Wang, Leping ³ ; Cao, Yajie ³ ; Li, Ziyong ¹ ; Wu, Yuxing ³ ; Tan, Yizhou ³ ; Shi, Yan ³ ; Zhang, Lili ² ; Liu, Hui ³ ; Li, Yinan ¹ ; Peng, Hao ³ ; Li, Xun ¹ ; Hu, Chuanhuo ¹ ; Wang, Xiaoye ¹ ;

作者机构： 1.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Guangxi, Peoples R China

2.Guangxi Zhuang Autonomous Reg Engn Res Ctr Vet Bio, Nanning 530004, Guangxi, Peoples R China

3.Guangxi Vet Res Inst, Nanning 530004, Guangxi, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Peoples R China

5.Guangxi Coll & Univ, Key Lab Prevent & Control Anim Dis, Nanning, Peoples R China

6.Guangxi Key Lab Livestock & Poultry Breeding & Dis, Liuzhou, Peoples R China

关键词： Oral; Microencapsulated phage; Prophylaxis; Escherichia coli; Salmonella Typhimurium

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2024 年 288 卷

页码：

收录情况： SCI

摘要： Escherichia coli and Salmonella Typhimurium are the main pathogens of diarrhea in weaned piglets. The pre-vention of bacterial diarrhea in weaned piglets by phage is rarely reported. We conducted this study to evaluate the preventive effect of phages on mixed Escherichia coli and Salmonella Typhimurium infections in weaned piglets. A novel phage named NJ12 was isolated by using Salmonella Typhimurium SM022 as host bacteria and characterized by electron microscopy, genomic analysis and in vitro bacteriostatic activity. Phage NJ12 and a previously reported phage EP01 were microencapsulated with sodium alginate to make phage cocktail. Micro-encapsulated phage cocktail and PBS (Phosphate buffer solution) were used to piglets the phage and phage-free group through oral administration before bacterial infection 2 h, respectively. Piglets of the phage and phage-free group were consumed with feed contaminated with 6 mL (108CFU/mL) Escherichia coli O157:H7 GN07 (GXEC-N07) and 6 mL (108CFU/mL) SM022 every day for seven consecutive days. The results showed that piglets in the phage-free group had more severe diarrhea, larger decreased average weight gain and higher levels of neutro-phils compared with piglets in phage group. Meanwhile, piglets in the phage-free group had higher load of SM022 and GN07 in jejunal tissue and more severe intestinal damage compared with piglets in group phage in vivo. In addition, oral administration phage can significant decreased the relative abundance of Enterobacteri-aceae but hardly repaired the changes of diversity and composition of gut microbiota caused by the mixed infection of SM022 and GN07. This implies that phage used as a feed additive have a marvelous preventive effect on bacterial diarrhea during weaning of piglets.