您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evolution and transmission potential of iuc3-positive virulence plasmids in hypervirulent Klebsiella pneumoniae

文献类型： 外文期刊

作者： Wang, Jiayun 1 ; Ji, Xing 2 ; Garcia, Pilar 3 ; Li, Jun 2 ; Zhang, Lili 2 ; Wang, Heng 1 ; Wang, Ran 2 ; He, Tao 2 ;

作者机构： 1.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control, Important Anim Infect Dis & Zoonoses, Yangzhou 225009, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Inst Food Safety & Nutr, Nanjing 210040, Peoples R China

3.Inst Productos Lacteos Asturias, Carretera Infiesto S-N, Asturias 33300, Spain

关键词： Klebsiella pneumoniae; HvKP; iucABCD; Genomic evolution; Conjugation; Plasmid fusion

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.9； 五年影响因子：7.2 ）

ISSN： 0944-5013

年卷期： 2025 年 299 卷

页码：

收录情况： SCI

摘要： The global emergence of hypervirulent Klebsiella pneumoniae (hvKP) poses significant threats to public health. The iucABCD (iuc) operon, a plasmid-borne genetic determinant encoding the siderophore aerobactin, serves as a reliable biomarker for hvKP identification. This study aimed to characterize the iuc3 (type 3 iuc)-positive K. pneumoniae isolates from diseased cows as hvKP strains and to elucidate the contribution of iuc3-positive plasmids to the hypervirulence phenotype, the evolutionary pathways and the spreading risk of the iuc3-positive plasmids. Eight of 108 K. pneumoniae isolates from 763 cow milk samples contained the iuc3 operon, and all iuc3 operons were located on T4SS-bearing plasmids. Notably, these isolates showed hypervirulent phenotype compared to the iuc3 knockout and plasmid-cured strains, including severe tissue damage, high bacterial loads in typical organs and high mortality in animal models. Analysis of NCBI database showed iuc3-positive K. pneumoniae strains have been widely distributed across animal, food, and humans, and exhibit an independent evolutionary pathways compared to the human-associated iuc1-type hvKP. The IS elements likely drive the acquisition of various resistance genes and the emergence of iuc3-positive resistance plasmids. The iuc3-bearing plasmids in this study demonstrated highly conjugative ability, low fitness costs, and the ability to replace the iuc1-bearing classical virulence plasmid in human hvKP strain. Besides, they could completely fuse with broad-host-range resistance plasmid (IncX3-blaNDM-5), becoming carbapenem-resistant hvKP strains. The iuc3-and blaNDM-5-fusion plasmids showed strong in vivo retention and dissemination capabilities in mouse models. Our findings emphasize the necessity for long-term monitoring of iuc3-positive plasmids in K. pneumoniae isolates from both animals and humans.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台